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TBX5 antibody

Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849
Anti-TBX5 antibody used in Western Blot (WB). GTX113849
Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849
Anti-TBX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113849
Anti-TBX5 antibody used in Western Blot (WB). GTX113849

Cat No. GTX113849

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HeLa nuclear extract , rat brain

Predict Reactivity

Mouse, Bovine, Chicken, Japanese Medaka, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBX5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

T-box 5,HOS

Cellular Localization

Nucleus

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849

GTX113849 IHC-P Image

TBX5 antibody detects TBX5 protein at nucleus on rat ovary by immunohistochemical analysis.
Sample: Paraffin-embedded rat ovary.
TBX5 antibody (GTX113849) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TBX5 antibody used in Western Blot (WB). GTX113849

GTX113849 WB Image

HeLa whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody (GTX124336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849

GTX113849 IHC-P Image

TBX5 antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
TBX5 antibody (GTX113849) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TBX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113849

GTX113849 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBX5(GTX113849) antibody at 1:500 dilution.

Anti-TBX5 antibody used in Western Blot (WB). GTX113849

GTX113849 WB Image

TBX5 antibody detects TBX5 protein by western blot analysis.
A. 50 µg rat brain lysate/extract
7.5% SDS-PAGE
TBX5 antibody (GTX113849) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169