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TBX5 antibody

Orthogonal
Anti-TBX5 antibody used in Western Blot (WB). GTX113849
Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849
Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849
Anti-TBX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113849
Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849
Anti-TBX5 antibody used in Western Blot (WB). GTX113849

Cat. No. GTX113849

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all TBX5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

HeLa nuclear extract

Predict Reactivity

Mouse, Bovine, Chicken, Japanese Medaka, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TBX5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038100

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T-box 5 , HOS

Cellular Localization

Nucleus

Background

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TBX5 antibody used in Western Blot (WB). GTX113849

GTX113849 WB Image

Zebrafish tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody (GTX113849) diluted at 1:1000.

Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849

GTX113849 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using TBX5 antibody (GTX113849) at 1:300 dilution.

Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849

GTX113849 IHC-P Image

TBX5 antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
TBX5 antibody (GTX113849) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TBX5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113849

GTX113849 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TBX5(GTX113849) antibody at 1:500 dilution.

Anti-TBX5 antibody used in IHC (Paraffin sections) (IHC-P). GTX113849

GTX113849 IHC-P Image

TBX5 antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat thyroid gland.
TBX5 antibody (GTX113849) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TBX5 antibody used in Western Blot (WB). GTX113849

GTX113849 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TBX5 antibody (GTX124336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Yin L et al. Stem Cell Res Ther 2022; 13 (1) : 324 RA signaling pathway combined with Wnt signaling pathway regulates human-induced pluripotent stem cells (hiPSCs) differentiation to sinus node-like cells.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169