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TC21 antibody [N1C3]

Anti-TC21 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX110559
Anti-TC21 antibody [N1C3] used in Western Blot (WB). GTX110559
Anti-TC21 antibody [N1C3] used in Western Blot (WB). GTX110559
Anti-TC21 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110559

Cat. No. GTX110559

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all TC21 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Mouse brain , A431

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TC21. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951791

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

RAS related 2 , TC21

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TC21 antibody [N1C3] used in IHC (Whole mount) (IHC-Wm). GTX110559

GTX110559 IHC-Wm Image

TC21 antibody [N1C3] detects TC21 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?
Sample: Paraformaldehyde-fixed zebrafish embryos.
TC21 antibody [N1C3] (GTX110559) dilution: 1:200.

Anti-TC21 antibody [N1C3] used in Western Blot (WB). GTX110559

GTX110559 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
12% SDS PAGE
GTX110559 diluted at 1:5000

Anti-TC21 antibody [N1C3] used in Western Blot (WB). GTX110559

GTX110559 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX110559 diluted at 1:5000

Anti-TC21 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX110559

GTX110559 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using TC21(GTX110559) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169