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TCEB1 antibody [HL1683]

Recombinant antibody KO/KD
Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282
Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282
Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282
Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282
Anti-TCEB1 antibody [HL1683] used in IHC (Paraffin sections) (IHC-P). GTX637282
Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

Cat. No. GTX637282

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1683

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Drosophila, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all TCEB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TCEB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

elongin C , SIII , TCEB1

Cellular Localization

Nucleus

Background

This gene encodes the protein elongin C, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

GTX637282 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (GTX637282) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

GTX637282 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (GTX637282) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

GTX637282 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (GTX637282) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

GTX637282 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (GTX637282) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCEB1 antibody [HL1683] used in IHC (Paraffin sections) (IHC-P). GTX637282

GTX637282 IHC-P Image

TCEB1 antibody [HL1683] detects TCEB1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
TCEB1 stained by TCEB1 antibody [HL1683] (GTX637282) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TCEB1 antibody [HL1683] used in Western Blot (WB). GTX637282

GTX637282 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TCEB1 antibody [HL1683] (GTX637282) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations