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TCF12 antibody

Anti-TCF12 antibody used in Western Blot (WB). GTX84997
Anti-TCF12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84997

Cat. No. GTX84997

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all TCF12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

Positive Control

HeLa Cell Slide(GTX25529) , HeLa Cell Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12.The immunogen is located within amino acids 50 - 100 of TCF12.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor 12 , CRS3 , HEB , HTF4 , HsT17266 , TCF-12 , bHLHb20 , p64

Cellular Localization

Nucleus

Background

TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.

Database

Research Area

DATA IMAGES

Anti-TCF12 antibody used in Western Blot (WB). GTX84997

GTX84997 WB Image

WB analysis of HeLa cell lysate using GTX84997 TCF12 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-TCF12 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84997

GTX84997 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84997 TCF12 antibody.
Working concentration : 10 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399