APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human TCF3 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75
Cellular Localization
Nucleus
Background
This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of c
Database
Research Area
DATA IMAGES
![Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355](/templates/default/js/lightbox/images/loading.gif "Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355")
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GTX83355 FACS Image
FACS analysis of A549 cells using GTX83355 TCF3 / E2A antibody [6B8].
Green : TCF3
Purple : negative control
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GTX83355 WB Image
WB analysis of A549 (1), A431 (2), HeLa (3), PANC-1 (4) and PC-3 (5) cell lysate using GTX83355 TCF3 / E2A antibody [6B8].
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REFERENCE
There are currently no references for TCF3 / E2A antibody [6B8] (GTX83355). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TCF3 / E2A antibody [6B8] (GTX83355). Be the first to share your experience with this product.
![Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355](/upload/website/prouct_img/small/GTX83355/GTX83355_20170912_FACS_w_23061322_378.webp "Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355")
![Anti-TCF3 / E2A antibody [6B8] used in Western Blot (WB). GTX83355](/upload/website/prouct_img/small/GTX83355/GTX83355_20170912_WB_w_23061322_797.webp "Anti-TCF3 / E2A antibody [6B8] used in Western Blot (WB). GTX83355")
![Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355](/upload/website/prouct_img/normal/GTX83355/GTX83355_20170912_FACS_w_23061322_378.webp "Anti-TCF3 / E2A antibody [6B8] used in Flow cytometry (FACS). GTX83355")
![Anti-TCF3 / E2A antibody [6B8] used in Western Blot (WB). GTX83355](/upload/website/prouct_img/normal/GTX83355/GTX83355_20170912_WB_w_23061322_797.webp "Anti-TCF3 / E2A antibody [6B8] used in Western Blot (WB). GTX83355")