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TCF3 / E2A antibody

Anti-TCF3 / E2A antibody used in Immunoprecipitation (IP). GTX109885
Anti-TCF3 / E2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109885
Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX109885
Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX109885
Anti-TCF3 / E2A antibody used in IHC (Paraffin sections) (IHC-P). GTX109885

Cat. No. GTX109885

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , NCI-H929 , BCL-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11172469

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75

Cellular Localization

Nucleus

Background

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TCF3 / E2A antibody used in Immunoprecipitation (IP). GTX109885

GTX109885 IP Image

TCF3 / E2A antibody immunoprecipitates TCF3 protein in IP experiments.
IP samples: Jurkat whole cell extract
A. 35 μg Jurkat whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of TCF3 protein by 4 μg TCF3 / E2A antibody (GTX109885)
7.5 % SDS-PAGE
The immunoprecipitated TCF3 protein was detected by TCF3 / E2A antibody (GTX109885) diluted at 1:1000.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-TCF3 / E2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109885

GTX109885 ICC/IF Image

TCF3 / E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 stained by TCF3 / E2A antibody (GTX109885) diluted at 1:600.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX109885

GTX109885 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody (GTX109885) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX109885

GTX109885 WB Image

Sample (30 ug of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX109885 diluted at 1:2000

Anti-TCF3 / E2A antibody used in IHC (Paraffin sections) (IHC-P). GTX109885

GTX109885 IHC-P Image

TCF3 / E2A antibody detects TCF3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TCF3 stained by TCF3 / E2A antibody (GTX109885) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169