GeneTex

  • Country / Location Selection

United States (US)

TCF3 / E2A antibody

Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX110458
Anti-TCF3 / E2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110458

Cat. No. GTX110458

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TCF3 / E2A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885285

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor 3 , AGM8 , E2A , E47 , ITF1 , TCF-3 , VDIR , bHLHb21 , p75

Cellular Localization

Nucleus

Background

The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TCF3 / E2A antibody used in Western Blot (WB). GTX110458

GTX110458 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCF3 / E2A antibody (GTX110458) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCF3 / E2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110458

GTX110458 ICC/IF Image

TCF3 / E2A antibody detects TCF3 protein at nucleus by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TCF3 protein stained by TCF3 / E2A antibody (GTX110458) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

There are currently no references for TCF3 / E2A antibody (GTX110458). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for TCF3 / E2A antibody (GTX110458). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169