APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 400-500 of human TCF4 (NP_001077431.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transcription factor 4 , E2-2 , FECD3 , ITF-2 , ITF2 , PTHS , SEF-2 , SEF2 , SEF2-1 , SEF2-1A , SEF2-1B , SEF2-1D , TCF-4 , bHLHb19
Cellular Localization
Nucleus
Background
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]
Database
Research Area
DATA IMAGES
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GTX16385 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX16385 TCF4 antibody.
Blue : DAPI
Dilution : 1:100
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GTX16385 ICC/IF Image
ICC/IF analysis of C6 cells using GTX16385 TCF4 antibody.
Blue : DAPI
Dilution : 1:100
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GTX16385 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX16385 TCF4 antibody.
Blue : DAPI
Dilution : 1:100
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GTX16385 WB Image
WB analysis of various sample lysates using GTX16385 TCF4 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
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REVIEW
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