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TCIRG1 antibody

Anti-TCIRG1 antibody used in Western Blot (WB). GTX32087

Cat No. GTX32087

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

EL4 Cell Lysate(GTX27183)

Specificity/Sensitivity

At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.The immunogen is located within amino acids 70 - 120 of TCIRG1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

T cell immune regulator 1, ATPase H+ transporting V0 subunit a3 , ATP6N1C , ATP6V0A3 , Atp6i , OC-116kDa , OC116 , OPTB1 , Stv1 , TIRC7 , Vph1 , a3

Cellular Localization

Membrane

Background

Through alternate splicing, this gene encodes two proteins with similarity to subunits of the vacuolar ATPase (V-ATPase) but the encoded proteins seem to have different functions. V-ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-TCIRG1 antibody used in Western Blot (WB). GTX32087

GTX32087 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32087 TCIRG1 antibody.
Working concentration : 0.5 μg/ml

REFERENCE

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Package List Price ($)
$ 329