GeneTex
United States (US)

TCIRG1 antibody

GTX32087_3214_WB_20180221_18121410_457.jpg

Cat No. GTX32087

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

93 kDa. ( Note )

Positive Control

EL4 Cell Lysate(GTX27183)

Specificity/Sensitivity

At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.The immunogen is located within amino acids 70 - 120 of TCIRG1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

T Cell Immune Regulator 1, Atpase H+ Transporting V0 Subunit A3,Atp6N1C,Atp6V0A3,Atp6I,Oc-116Kda,Oc116,Optb1,Stv1,Tirc7,Vph1,A3,Tcirg1

Background

Through alternate splicing, this gene encodes two proteins with similarity to subunits of the vacuolar ATPase (V-ATPase) but the encoded proteins seem to have different functions. V-ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX32087_3214_WB_20180221_18121410_457.jpg

GTX32087 WB Image

WB analysis of EL4 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32087 TCIRG1 antibody.
Working concentration : 0.5 μg/ml

Package List Price ($)
$ 329