APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-2μg/ml |
| 1-2μg/ml |
|
Note :
IHC-P
Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX10180-PEP).
Product Note
This antibody is expected to recognise both reported isoforms.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2; NP_001008897.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
t-complex 1 , CCT-alpha , CCT1 , CCTa , D6S230E , TCP-1-alpha
Cellular Localization
Cytoplasm, cytosol
Background
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]
Database
Research Area
DATA IMAGES
|
GTX10180 WB Image
WB analysis of human lung lysate using GTX10180 TCP1 alpha antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX10180 IHC-P Image
IHC-P analysis of human testis using GTX10180 TCP1 alpha antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10μg/ml
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REFERENCE
There are currently no references for TCP1 alpha antibody, C-term (GTX10180). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TCP1 alpha antibody, C-term (GTX10180). Be the first to share your experience with this product.
