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TCP1 alpha antibody, C-term

Anti-TCP1 alpha antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10180
Anti-TCP1 alpha antibody, C-term used in Western Blot (WB). GTX10180

Cat No. GTX10180

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-2μg/ml
IHC-P 1-2μg/ml

Note :

IHC-P
Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli.

Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX10180-PEP).

Specificity/Sensitivity

This antibody is expected to recognise both reported isoforms.
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2; NP_001008897.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

T-Complex 1 , Cct-Alpha , Cct1 , Ccta , D6S230E , Tcp-1-Alpha , Tcp1

Cellular Localization

Cytoplasmic

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES
Anti-TCP1 alpha antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX10180

GTX10180 IHC-P Image

IHC-P analysis of human testis using GTX10180 TCP1 alpha antibody, C-term.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 10µg/ml

Anti-TCP1 alpha antibody, C-term used in Western Blot (WB). GTX10180

GTX10180 WB Image

WB analysis of human lung lysate using GTX10180 TCP1 alpha antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

REFERENCE
There are currently no references for TCP1 alpha antibody, C-term (GTX10180). Be the first to share your publications with this product.
REVIEW
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Package List Price ($)
$ 319