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TCP1 alpha antibody

Anti-TCP1 alpha antibody used in Western Blot (WB). GTX16399
Anti-TCP1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX16399
Anti-TCP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16399

Cat. No. GTX16399

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all TCP1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

t-complex 1 , CCT-alpha , CCT1 , CCTa , D6S230E , TCP-1-alpha

Cellular Localization

Cytoplasm, cytosol

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-TCP1 alpha antibody used in Western Blot (WB). GTX16399

GTX16399 WB Image

WB analysis of various sample lysates using GTX16399 TCP1 alpha antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-TCP1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX16399

GTX16399 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX16399 TCP1 alpha antibody.
Dilution : 1:100

Anti-TCP1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX16399

GTX16399 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX16399 TCP1 alpha antibody.
Blue : DAPI

REFERENCE

Application Reference

Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399