APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human TCP1 beta expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chaperonin containing TCP1 subunit 2 , 99D8.1 , CCT-beta , CCTB , HEL-S-100n , PRO1633 , TCP-1-beta
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Database
Research Area
DATA IMAGES
![Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505](/templates/default/js/lightbox/images/loading.gif "Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505")
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GTX60505 WB Image
WB analysis of human TCP1 (AA: 87-290) recombinant protein using GTX60505 TCP1 beta antibody [5B5C4].
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GTX60505 ELISA Image
ELISA analysis of antigen using GTX60505 TCP1 beta antibody [5B5C4].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60505 ICC/IF Image
ICC/IF analysis of 3T3-L1 cells using GTX60505 TCP1 beta antibody [5B5C4].
Green : TCP1 beta
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60505 FACS Image
FACS analysis of NIH3T3 cells using GTX60505 TCP1 beta antibody [5B5C4].
Blue : TCP1 beta
Red : negative control
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GTX60505 WB Image
WB analysis of HeLa (1), MCF-7 (2), Jurkat (3), T47D (4), K562 (5), A431 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60505 TCP1 beta antibody [5B5C4].
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REFERENCE
There are currently no references for TCP1 beta antibody [5B5C4] (GTX60505). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TCP1 beta antibody [5B5C4] (GTX60505). Be the first to share your experience with this product.
![Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505](/upload/website/prouct_img/small/GTX60505/GTX60505_20170912_WB_1_w_23061123_137.webp "Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in ELISA (ELISA). GTX60505](/upload/website/prouct_img/small/GTX60505/GTX60505_20170912_ELISA_w_23061123_952.webp "Anti-TCP1 beta antibody [5B5C4] used in ELISA (ELISA). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60505](/upload/website/prouct_img/small/GTX60505/GTX60505_20170912_ICCIF_w_23061123_640.webp "Anti-TCP1 beta antibody [5B5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Flow cytometry (FACS). GTX60505](/upload/website/prouct_img/small/GTX60505/GTX60505_20170912_FACS_w_23061123_737.webp "Anti-TCP1 beta antibody [5B5C4] used in Flow cytometry (FACS). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505](/upload/website/prouct_img/small/GTX60505/GTX60505_20170912_WB_w_23061123_706.webp "Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505](/upload/website/prouct_img/normal/GTX60505/GTX60505_20170912_WB_1_w_23061123_137.webp "Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in ELISA (ELISA). GTX60505](/upload/website/prouct_img/normal/GTX60505/GTX60505_20170912_ELISA_w_23061123_952.webp "Anti-TCP1 beta antibody [5B5C4] used in ELISA (ELISA). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60505](/upload/website/prouct_img/normal/GTX60505/GTX60505_20170912_ICCIF_w_23061123_640.webp "Anti-TCP1 beta antibody [5B5C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Flow cytometry (FACS). GTX60505](/upload/website/prouct_img/normal/GTX60505/GTX60505_20170912_FACS_w_23061123_737.webp "Anti-TCP1 beta antibody [5B5C4] used in Flow cytometry (FACS). GTX60505")
![Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505](/upload/website/prouct_img/normal/GTX60505/GTX60505_20170912_WB_w_23061123_706.webp "Anti-TCP1 beta antibody [5B5C4] used in Western Blot (WB). GTX60505")