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TCP1 beta antibody

Anti-TCP1 beta antibody used in Immunoprecipitation (IP). GTX33535
Anti-TCP1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33535
Anti-TCP1 beta antibody used in Western Blot (WB). GTX33535

Cat. No. GTX33535

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all TCP1 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 206-535 of human CCT2 (NP_006422.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chaperonin containing TCP1 subunit 2 , 99D8.1 , CCT-beta , CCTB , HEL-S-100n , PRO1633 , TCP-1-beta

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-TCP1 beta antibody used in Immunoprecipitation (IP). GTX33535

GTX33535 IP Image

IP analysis of A549 cell lysate using GTX33535 TCP1 beta antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-TCP1 beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33535

GTX33535 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33535 TCP1 beta antibody.
Blue : DAPI

Anti-TCP1 beta antibody used in Western Blot (WB). GTX33535

GTX33535 WB Image

WB analysis of various sample lysates using GTX33535 TCP1 beta antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399