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TCP1 epsilon antibody [GT639]

KO/KD Orthogonal
Anti-TCP1 epsilon antibody [GT639] used in Western Blot (WB). GTX631668
Anti-TCP1 epsilon antibody [GT639] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631668
Anti-TCP1 epsilon antibody [GT639] used in Western Blot (WB). GTX631668
Anti-TCP1 epsilon antibody [GT639] used in Immunoprecipitation (IP). GTX631668

Cat. No. GTX631668

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT639

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human, Hamster
Package
100 μl ($399),
25 μl ($169)
See all TCP1 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 epsilon. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888252

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chaperonin containing TCP1 subunit 5 , CCT-epsilon , CCTE , HEL-S-69 , PNAS-102 , TCP-1-epsilon

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-TCP1 epsilon antibody [GT639] used in Western Blot (WB). GTX631668

GTX631668 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000.

Anti-TCP1 epsilon antibody [GT639] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631668

GTX631668 ICC/IF Image

TCP1 epsilon antibody [GT639] detects TCP1 epsilon protein at centrosome and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: TCP1 epsilon stained by TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-TCP1 epsilon antibody [GT639] used in Western Blot (WB). GTX631668

GTX631668 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-TCP1 epsilon antibody [GT639] used in Immunoprecipitation (IP). GTX631668

GTX631668 IP Image

Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [GT639] (GTX631668).
Western blot analysis was performed using TCP1 epsilon antibody [GT639] (GTX631668).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE

Application Reference

Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Rawad Hodeify et al. Science Advances 2018; 4 : 9 The CCT chaperonin is a novel regulator of Ca2+ signaling through modulation of Orai1 trafficking.
Application : IP
Reactivity : Hamster
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations