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TCP1 epsilon antibody [N1C1]

KO/KD
Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in Immunoprecipitation (IP). GTX110167
Anti-TCP1 epsilon antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX110167

Cat. No. GTX110167

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all TCP1 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Mouse brain , Molt-4 , 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949910

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chaperonin containing TCP1 subunit 5 , CCT-epsilon , CCTE , HEL-S-69 , PNAS-102 , TCP-1-epsilon

Cellular Localization

Cytoplasm

Background

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167

GTX110167 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1C1] (GTX110167) diluted at 1:6000.

Anti-TCP1 epsilon antibody [N1C1] used in IHC (Frozen sections) (IHC-Fr). GTX110167

GTX110167 IHC-Fr Image

Immunohistochemical analysis of frozen sections of zebrafish muscle, 5 hpf, using TCP1 epsilon antibody [N1C1] (GTX110167) at 1:100 dilution.

Anti-TCP1 epsilon antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110167

GTX110167 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon(GTX110167) antibody at 1:200 dilution.

Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167

GTX110167 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX110167 diluted at 1:1000

Anti-TCP1 epsilon antibody [N1C1] used in Western Blot (WB). GTX110167

GTX110167 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX110167 diluted at 1:10000

Anti-TCP1 epsilon antibody [N1C1] used in Immunoprecipitation (IP). GTX110167

GTX110167 IP Image

Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [N1C1] (GTX110167).
Western blot analysis was performed using TCP1 epsilon antibody [N1C1] (GTX110167).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TCP1 epsilon antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX110167

GTX110167 IHC-P Image

TCP1 epsilon antibody [N1C1] detects TCP1 epsilon protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TCP1 epsilon antibody [N1C1] (GTX110167) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Berger J et al. PLoS Genet 2022; 18 (6) : e1010287 Mob4-dependent STRIPAK involves the chaperonin TRiC to coordinate myofibril and microtubule network growth.
Reactivity : Zebrafish
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169