APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
| 5μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88407-PEP).
Product Note
This antibody is expected to recognize all reported isoforms (NP_005989.3; NP_001008883.1; NP_001008800.1).
Predict Reactivity
Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-GHKKKGDDQSRQGG, from the C-Terminus (near) of the protein sequence according to NP_005989.3; NP_001008883.1; NP_001008800.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
chaperonin containing TCP1 subunit 3 , CCT-gamma , CCTG , PIG48 , TCP-1-gamma , TRIC5
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants have been characterized for this gene. In addition, a pseudogene of this gene has been found on chromosome 8. [provided by RefSeq, Aug 2010]
Database
Research Area
DATA IMAGES
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GTX88407 WB Image
WB analysis of mouse testis lysate using GTX88407 TCP1 gamma antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88407 IHC-P Image
IHC-P analysis of human tonsil using GTX88407 TCP1 gamma antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5μg/ml
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REFERENCE
There are currently no references for TCP1 gamma antibody, C-term (GTX88407). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TCP1 gamma antibody, C-term (GTX88407). Be the first to share your experience with this product.
