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TCP1 theta antibody [N2C2], Internal

Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

Cat No. GTX105725

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

chaperonin containing TCP1 subunit 8 , C21orf112 , Cctq , D21S246 , PRED71

Cellular Localization

Cytoplasm

Background

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Database

DATA IMAGES
Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

GTX105725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using TCP-1 theta (GTX105725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725

GTX105725 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
C: Hela
7.5% SDS PAGE
GTX105725 diluted at 1:1000

Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725

GTX105725 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105725 diluted at 1:5000

Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

GTX105725 IHC-P Image

TCP1 theta antibody [N2C2], Internal detects TCP1 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TCP1 theta antibody [N2C2], Internal (GTX105725) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169