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TCP1 theta antibody [N2C2], Internal

Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725
Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

Cat. No. GTX105725

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TCP1 theta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036474

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

chaperonin containing TCP1 subunit 8 , C21orf112 , Cctq , D21S246 , PRED71

Cellular Localization

Cytoplasm

Background

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.

Database

Research Area

DATA IMAGES

Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725

GTX105725 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 theta antibody [N2C2], Internal (GTX105725) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

GTX105725 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using TCP-1 theta (GTX105725) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TCP1 theta antibody [N2C2], Internal used in Western Blot (WB). GTX105725

GTX105725 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX105725 diluted at 1:5000

Anti-TCP1 theta antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX105725

GTX105725 IHC-P Image

TCP1 theta antibody [N2C2], Internal detects TCP1 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TCP1 theta antibody [N2C2], Internal (GTX105725) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Wei PL et al. Tumour Biol 2015; (Epub) Glucose-regulated protein 94 mediates metastasis by CCT8 and the JNK pathway in hepatocellular carcinoma.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169