GeneTex
United States (US)

TCP1 theta antibody

GTX115466_40464_IFA_18121410_960.jpg
GTX115466_40464_WB_18121410_847.jpg

Cat No. GTX115466

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.99mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCP1 theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C21orf112 antibody, Cctq antibody, D21S246 antibody, KIAA0002 antibody, PRED71 antibody, CCT8 antibody, T-complex protein 1 subunit theta antibody, TCP-1-theta antibody, renal carcinoma antigen NY-REN-15 antibody, "T-complex protein 1, theta subunit antibody", CCT-theta antibody, "chaperonin containing TCP1, subunit 8 (theta) antibody"

Cellular Localization

Cytoplasm

Background

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.
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Database

DATA IMAGES
GTX115466_40464_IFA_18121410_960.jpg

GTX115466 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using TCP1 theta(GTX115466) antibody at 1:500 dilution.

GTX115466_40464_WB_18121410_847.jpg

GTX115466 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX115466 diluted at 1:10000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169