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TCPTP antibody

Anti-TCPTP antibody used in Immunoprecipitation (IP). GTX130319
Anti-TCPTP antibody used in Western Blot (WB). GTX130319
Anti-TCPTP antibody used in IHC (Paraffin sections) (IHC-P). GTX130319

Cat No. GTX130319

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:571
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TCPTP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 2 , PTN2 , PTPT , TC-PTP , TCELLPTP , TCPTP

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-TCPTP antibody used in Immunoprecipitation (IP). GTX130319

GTX130319 IP Image

Immunoprecipitation of TCPTP protein from A431 whole cell extracts using 5 μg of TCPTP antibody (GTX130319).
Western blot analysis was performed using TCPTP antibody (GTX130319).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TCPTP antibody used in Western Blot (WB). GTX130319

GTX130319 WB Image

TCPTP antibody detects TCPTP protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TCPTP antibody (GTX130319) diluted at a dilution of 1:1000.

Anti-TCPTP antibody used in IHC (Paraffin sections) (IHC-P). GTX130319

GTX130319 IHC-P Image

TCPTP antibody detects TCPTP protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TCPTP antibody (GTX130319) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for TCPTP antibody (GTX130319). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169