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TCR V beta 5b antibody [W112] (FITC)

Anti-TCR V beta 5b antibody [W112] (FITC) used in Flow cytometry (FACS). GTX79228

Cat. No. GTX79228

Host

Mouse

Clonality

Monoclonal

Clone Name

W112

Isotype

IgG1

Application

WB, IHC-Fr, FACS, IP, Activation

Reactivity

Human
Package
200 test ($489)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS 1-2 μg/test
IP Assay dependent
Activation Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. Protect from light.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human TCR Vb5.3

Purification

Protein G purified

Conjugation

Fluorescein isothiocyanate (FITC) Wavelength

RRID

AB_11168591

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

T cell receptor beta locus , TCRB , TRB@

Background

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TCR V beta 5b antibody [W112] (FITC) used in Flow cytometry (FACS). GTX79228

GTX79228 FACS Image

FACS analysis of positive HPB-ALL cells (left and middle panel) or negative control Jurkat cells (right panel) using GTX79228 TCR V beta 5b antibody [W112] (FITC).
Dilution : 5ul of primary antibody were used per test

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489