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TCR V delta 1 antibody [TS8.2]

Anti-TCR V delta 1 antibody [TS8.2] used in Flow cytometry (FACS). GTX79232

Cat. No. GTX79232

Host

Mouse

Clonality

Monoclonal

Clone Name

TS8.2

Isotype

IgG1

Application

ICC/IF, IHC-Fr, FACS, ELISA, Activation, IHC, Neutralizing/Inhibition

Reactivity

Human, Primate
Package
200 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS 1-2 μg/test
ELISA Assay dependent
Activation Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human TCR V delta 1

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_11175862

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

T cell receptor delta variable 1 , hDV101S1

Background

T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-TCR V delta 1 antibody [TS8.2] used in Flow cytometry (FACS). GTX79232

GTX79232 FACS Image

FACS analysis of positive PEER cells (left and middle panel) or negative control Jurkat cells (right panel) using GTX79232 TCR V delta 1 antibody [TS8.2].
Dilution : 5ul of primary antibody were used per test

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399