For FACS: Use at an assay dependent dilution. For IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.
Detects a T cell-specific heterodimeric 40 and 46 kD cell surface protein that is expressed on the vast majority, but not all, peripheral rat T cells. Induces blastoid transformation and IL2R expression in T-cells when cross linked on an artificial surface. It also induces cell proliferation when exogenous IL-2 is added.
Phosphate-buffered saline containing 0.02% sodium azide and BSA
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Rat T Blasts
Protein G purified
Fluorescein isothiocyanate (FITC)
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
RATTCBC1, MGC109620, TCR alpha + beta, TCB, RATTCB, Tcrb, TCBC1, 24820
The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.