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TCR alpha + TCR beta antibody [R73] (Low endotoxin, azide free)

Anti-TCR alpha + TCR beta antibody [R73] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX29314

Cat. No. GTX29314

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 R73

Isotype

 IgG1

Application

 IHC-Fr, FACS, IP, Activation

Reactivity

 Rat, Monkey, Cynomolgus monkey
Package
125 μg ($399)
See all TCR alpha + TCR beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr 1/10-1/100
FACS 1/50-1/100
IP Assay dependent
Activation Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.

Activation
Mouse anti Rat TCR alpha/beta antibody, clone R73 is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010).
Not tested in other applications.

Product Note

This antibody recognizes a constant determinant on the beta chain of the rat alpha/beta T cell receptor.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat T Blasts and Erythrocytes.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-TCR alpha + TCR beta antibody [R73] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX29314

GTX29314 FACS Image

FACS analysis of rat spleen cells using GTX29314 TCR alpha + beta antibody [R73] (Low endotoxin, azide free).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations