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TDG antibody [GT1142]

Anti-TDG antibody [GT1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631934
Anti-TDG antibody [GT1142] used in Western Blot (WB). GTX631934

Cat. No. GTX631934

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1142

Isotype

 IgG2b

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TDG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

human TDG -transfected 293T cells

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888261

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymine DNA glycosylase , hTDG

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDG antibody [GT1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631934

GTX631934 ICC/IF Image

TDG antibody [GT1142] detects TDG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDG protein stained by TDG antibody [GT1142] (GTX631934) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-TDG antibody [GT1142] used in Western Blot (WB). GTX631934

GTX631934 WB Image

TDG antibody detects TDG protein by western blot analysis. Non-transfected (-) and TDG -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX631934) diluted by 1:2500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations