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TDG antibody

KO/KD Overexpression
Anti-TDG antibody used in Western Blot (WB). GTX110473
Anti-TDG antibody used in Western Blot (WB). GTX110473
Anti-TDG antibody used in Western Blot (WB). GTX110473
Anti-TDG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110473

Cat. No. GTX110473

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TDG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720524

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

thymine DNA glycosylase , hTDG

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

KO/KD

Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 26917720

Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500.

Anti-TDG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110473

GTX110473 ICC/IF Image

TDG antibody detects TDG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDG protein stained by TDG antibody (GTX110473) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Mol Cell. 2021; 81 (14) : 2960-2974 UHRF2 commissions the completion of DNA demethylation through allosteric activation by 5hmC and K33-linked ubiquitination of XRCC1.
Application : WB
Reactivity : Human
Someda M et al. Cell Death Differ 2019; (Epub) Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis.
Application : WB
Reactivity : Mouse
McLaughlin D et al. J Biol Chem 2016; 291 (17) : 9014-24 Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo.
Application : WB
Reactivity : Human
Jin C et al. Epigenetics 2015; 10 (11) : 1006-13 Minimal role of base excision repair in TET-induced global DNA demethylation in HEK293T cells.
Xu X et al. J Biol Chem 2014; 289 (13) : 8881-90 Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169