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TDG antibody

KO/KD Overexpression
Anti-TDG antibody used in Western Blot (WB). GTX110473
Anti-TDG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110473
Anti-TDG antibody used in Western Blot (WB). GTX110473
Anti-TDG antibody used in Western Blot (WB). GTX110473

Cat No. GTX110473

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($379),
25 μl ($169)
See all TDG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa , Jurkat , Raji , NCI-H929 , TDG-transfected 293T

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10720524

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

thymine DNA glycosylase , hTDG

Cellular Localization

Nucleus

Background

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

KO/KD

Wild-type (WT) and TDG knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110473

GTX110473 ICC/IF Image

TDG antibody detects TDG protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDG protein stained by TDG antibody (GTX110473) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDG antibody used in Western Blot (WB). GTX110473

GTX110473 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:500.

REFERENCE

Application Reference

Mol Cell. 2021; 81 (14) : 2960-2974 UHRF2 commissions the completion of DNA demethylation through allosteric activation by 5hmC and K33-linked ubiquitination of XRCC1.
Application : WB
Reactivity : Human
Someda M et al. Cell Death Differ 2019; (Epub) Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis.
Application : WB
Reactivity : Mouse
McLaughlin D et al. J Biol Chem 2016; 291 (17) : 9014-24 Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo.
Application : WB
Reactivity : Human
Jin C et al. Epigenetics 2015; 10 (11) : 1006-13 Minimal role of base excision repair in TET-induced global DNA demethylation in HEK293T cells.
Xu X et al. J Biol Chem 2014; 289 (13) : 8881-90 Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169