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TDP43 antibody [GT225]

Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196
Anti-TDP43 antibody [GT225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630196
Anti-TDP43 antibody [GT225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630196

Cat No. GTX630196

Host

Mouse

Clonality

Monoclonal

Clone Name

GT225

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , HeLa , NIH-3T3 , Raw264.7 , PC-12 , Rat2 , 293T , TARDBP shRNA-transfected 293T , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888198

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196

GTX630196 IHC-P Image

TDP43 antibody [GT225] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT225] GTX630196
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:3000
GAPDH antibody (GTX100118) dilution: 1:10000

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Anti-TDP43 antibody [GT225] used in IHC (Paraffin sections) (IHC-P). GTX630196

GTX630196 IHC-P Image

TDP43 antibody [GT225] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT225] (GTX630196) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg BCL-1 whole cell lysate/extract
B. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

TARDBP antibody [GT225] detects TARDBP protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT225] (GTX630196) dilution: 1:1000

Anti-TDP43 antibody [GT225] used in Western Blot (WB). GTX630196

GTX630196 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT225] (GTX630196) diluted at 1:500.

Anti-TDP43 antibody [GT225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630196

GTX630196 ICC/IF Image

TDP43 antibody [GT225] detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133112) diluted at 1:500.
Red: TDP43 stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TDP43 antibody [GT225] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630196

GTX630196 ICC/IF Image

TDP43 antibody [GT225] detects TDP43 protein immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nestin stained by Nestin antibody (GTX133111) diluted at 1:500.
Red: TDP43 stained by TDP43 antibody [GT225] (GTX630196) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169