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TDP43 antibody [GT6310]

Anti-TDP43 antibody [GT6310] used in Western Blot (WB). GTX633973
Anti-TDP43 antibody [GT6310] used in IHC (Frozen sections) (IHC-Fr). GTX633973
Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973
Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973
Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973

Cat. No. GTX633973

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT6310

Isotype

 IgG2a

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TDP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888400

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody [GT6310] used in Western Blot (WB). GTX633973

GTX633973 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT6310] (GTX633973) diluted at 1:5000.

Anti-TDP43 antibody [GT6310] used in IHC (Frozen sections) (IHC-Fr). GTX633973

GTX633973 IHC-Fr Image

TDP43 antibody [GT6310] detects TDP43 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: TDP43 stained by TDP43 antibody [GT6310] (GTX633973) diluted at 1:250.
Red: Calbindin, stained by Calbindin antibody (GTX130856) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 5 min

Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973

GTX633973 IHC-P Image

TDP43 antibody [GT6310] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT6310] (GTX633973) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973

GTX633973 IHC-P Image

TDP43 antibody [GT6310] detects TDP43 protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
TDP43 antibody [GT6310] (GTX633973) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX633973

GTX633973 IHC-P Image

TDP43 antibody [GT6310] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT6310] (GTX633973) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Currently in production
Custom Conjugations