TDP43 antibody [GT733]

TDP43 antibody [GT733] detects TDP43 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody [GT733] (GTX630197) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

TARDBP antibody [GT733] detects TARDBP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT733] (GTX630197) dilution: 1:1000

TDP43 antibody [GT733] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT733] (GTX630197) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

TARDBP antibody [GT733] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT733] GTX630197
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT733] (GTX630197) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT733] (GTX630197) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

TDP43 antibody [GT733] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT733] (GTX630197) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT733] (GTX630197) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.