GeneTex
United States (US)

TDP43 antibody

KO/KD
GTX100579_39506_20160316_IHC-P_M_19011506_906.jpg
GTX100579_39506_IHC_R_19011506_112.jpg
GTX100579_39506_WB_shRNA_watermark_19011506_417.jpg
GTX100579_39511_IFA_19011506_914.jpg
GTX100579_WB_19011506_195.jpg
GTX100579_39506_WB_M_Brain_19011506_793.jpg

Cat No. GTX100579

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain

Predict Reactivity

Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALS10 antibody, TDP-43 antibody, TARDBP antibody, TAR DNA-binding protein 43 antibody, TAR DNA-binding protein-43 antibody, TAR DNA binding protein antibody

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100579_39506_20160316_IHC-P_M_19011506_906.jpg

GTX100579 IHC-P Image

TARDBP antibody detects TARDBP protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TARDBP antibody (GTX100579) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100579_39506_IHC_R_19011506_112.jpg

GTX100579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX100579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100579_39506_WB_shRNA_watermark_19011506_417.jpg

GTX100579 WB Image

KO/KD

Western blot analysis of TDP-43 (GTX100579, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (30 ug of whole cell lysate)
  A: HeLa mock control 
B: HeLa transfected shTDP-43
10% SDS PAGE 
GTX100579 diluted at 1:500

GTX100579_39511_IFA_19011506_914.jpg

GTX100579 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TARDBP(GTX100579) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX100579_WB_19011506_195.jpg

GTX100579 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
10% SDS PAGE
GTX100579 diluted at 1:1000

GTX100579_39506_WB_M_Brain_19011506_793.jpg

GTX100579 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX100579 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169