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TDP43 antibody

KO/KD
Anti-TDP43 antibody used in Western Blot (WB). GTX100579
Anti-TDP43 antibody used in Western Blot (WB). GTX100579
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX100579
Anti-TDP43 antibody used in Western Blot (WB). GTX100579
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX100579
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100579

Cat. No. GTX100579

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TDP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

H1299 , HeLa , mouse brain

Predict Reactivity

Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1241379

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody used in Western Blot (WB). GTX100579

GTX100579 WB Image

KO/KD

Western blot analysis of TDP-43 (GTX100579, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (30 ug of whole cell lysate)
  A: HeLa mock control 
B: HeLa transfected shTDP-43
10% SDS PAGE 
GTX100579 diluted at 1:500

Anti-TDP43 antibody used in Western Blot (WB). GTX100579

GTX100579 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX100579 diluted at 1:500

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX100579

GTX100579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX100579) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TDP43 antibody used in Western Blot (WB). GTX100579

GTX100579 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
10% SDS PAGE
GTX100579 diluted at 1:1000

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX100579

GTX100579 IHC-P Image

TARDBP antibody detects TARDBP protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TARDBP antibody (GTX100579) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100579

GTX100579 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TARDBP(GTX100579) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169