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TDP43 antibody

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210

Cat No. GTX114210

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP-seq
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP-seq Assay dependent

Note :

IP
target validated by Mass Spectrometry

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat-2 , Rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tar Dna Binding Protein,Als10,Tdp-43,Tardbp

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210

GTX114210 IP Image

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX114210 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

GTX114210 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TDP-43(GTX114210) antibody at 1:200 dilution.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX114210) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (30 µg of whole cell lysate)
  A: HeLa mock control 
B: HeLa transfected shTDP-43
10% SDS PAGE 
GTX114210 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169