APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
| Assay dependent |
|
Note :
IP
target validated by Mass Spectrometry
|
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat-2 , Rat brain
Predict Reactivity
Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.59 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038095
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
TAR DNA binding protein , ALS10 , TDP-43
Cellular Localization
Nucleus
Background
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX114210 IP Image
TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX114210 WB Image
TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114210 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX114210 ICC/IF Image
TDP43 antibody detects TDP43 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:2000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
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GTX114210 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114210 ICC/IF Image
TDP43 antibody detects TDP43 protein by immunofluorescent analysis.Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX114210 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114210 WB Image
Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel)
Sample (30 μg of whole cell lysate)
A: HeLa mock control
B: HeLa transfected shTDP-43
10% SDS PAGE
GTX114210 diluted at 1:1000.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX114210 IHC-P Image
TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX114210 IHC-P Image
TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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