GeneTex
United States (US)

TDP43 antibody

KO/KD
GTX114210_40135_IP_18121410_984.jpg
GTX114210_40135_WB_M_18121410_612.jpg
GTX114210_40135_WB_R_Brain_18121410_110.jpg
GTX114210_IHC_18121410_503.jpg
GTX114210_IFA_18121410_864.jpg
GTX114210_42851_20170511_WB_18121410_210.jpg
GTX114210_40135_IHC_R_18121410_913.jpg
GTX114210_40135_20170518_WB_R_18121410_458.jpg
GTX114210_40135_WB_shRNA_watermark_18121410_830.jpg

Cat No. GTX114210

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP-seq
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP-seq Assay dependent

Note :

IP
target validated by Mass Spectrometry

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat-2 , Rat brain

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.5mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ALS10 antibody, TDP-43 antibody, TARDBP antibody, TAR DNA-binding protein 43 antibody, TAR DNA-binding protein-43 antibody, TAR DNA binding protein antibody

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114210_40135_IP_18121410_984.jpg

GTX114210 IP Image

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX114210_40135_WB_M_18121410_612.jpg

GTX114210 WB Image

TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg NIH-3T3 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114210_40135_WB_R_Brain_18121410_110.jpg

GTX114210 WB Image

Sample (50 µg of whole cell lysate)
A: Rat brain
10% SDS PAGE
GTX114210 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114210_IHC_18121410_503.jpg

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114210_IFA_18121410_864.jpg

GTX114210 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TDP-43(GTX114210) antibody at 1:200 dilution.

GTX114210_42851_20170511_WB_18121410_210.jpg

GTX114210 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114210_40135_IHC_R_18121410_913.jpg

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded rat brain, using TARDBP(GTX114210) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114210_40135_20170518_WB_R_18121410_458.jpg

GTX114210 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114210_40135_WB_shRNA_watermark_18121410_830.jpg

GTX114210 WB Image

KO/KD

Western blot analysis of TDP-43 (GTX114210, upper panel) and beta-actin (GTX109639, lower panel) 
Sample (30 µg of whole cell lysate)
  A: HeLa mock control 
B: HeLa transfected shTDP-43
10% SDS PAGE 
GTX114210 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169