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TDP43 antibody

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

Cat. No. GTX114210

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP-seq, RIP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TDP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP-seq Assay dependent
RIP Assay dependent

Note :

IP
target validated by Mass Spectrometry
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat-2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038095

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210

GTX114210 IP Image

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

GTX114210 ICC/IF Image

TDP43 antibody detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

GTX114210 ICC/IF Image

TDP43 antibody detects TDP43 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

REFERENCE

Application Reference

Chu JF et al. Cell Rep 2019; 29 (10) : 3118-3133.e6 TDP-43 Regulates Coupled Dendritic mRNA Transport-Translation Processes in Co-operation with FMRP and Staufen1.
Application : ICC/IF, RIP
Reactivity : Mouse
Wu LS et al. Acta Neuropathol Commun 2019; 7 (1) : 50 Transcriptomopathies of pre- and post-symptomatic frontotemporal dementia-like mice with TDP-43 depletion in forebrain neurons.
Application : IHC-P
Reactivity : Mouse
Huang CY et al. Journal of Neuroimmunology 2017; : Epub ahead of print TDP-43 proteolysis is associated with astrocyte reactivity after traumatic brain injury in rodents.
Application : IHC-P
Majumder P et al. Acta Neuropathol 2016; 132 (5) : 721-738 Co-regulation of mRNA translation by TDP-43 and Fragile X Syndrome protein FMRP.
Application : WB, ICC/IF, IP
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169