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TDP43 antibody

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX30809
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30809
Anti-TDP43 antibody used in Western Blot (WB). GTX30809
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX30809
Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX30809

Cat. No. GTX30809

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA, IHC

Reactivity

 Human, Mouse
Package
150 μl ($439)
See all TDP43 products

APPLICATION

Application Note

For ELISA: Use at a dilution of 1:1,000-1:100,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:100-1:1,000. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

45 kDa. ( Note )

Positive Control

K562 cells, Glioma

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 50% Glycerol

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

667 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant TARDBP protein

Purification

Affinity purified with antigen

Conjugation

Unconjugated

RRID

AB_1952814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus , Cytoplasm

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX30809

GTX30809 IHC-P Image

Immunohistochemical of paraffin-embedded human lung cancer using GTX30809 (TARDBP antibody) at dilution of 1:100 (under 40x lens)

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30809

GTX30809 ICC/IF Image

Immunofluorescent analysis of A549 cells, using TARDBP antibody GTX30809 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Anti-TDP43 antibody used in Western Blot (WB). GTX30809

GTX30809 WB Image

Western blot analysis in HeLa cell extract using GTX30809 (TARDBP antibody) at a dilution of 1:1200

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX30809

GTX30809 IHC-P Image

Immunohistochemical of paraffin-embedded human lung cancer using GTX30809 (TARDBP antibody) at dilution of 1:100 (under 10x lens)

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX30809

GTX30809 IP Image

Immunoprecipitation result of anti-TARDBP (GTX30809, 3ug)
Detection: GTX30809 at 1:1000 dilution with HeLa cells lysate 3000ug.

REFERENCE

Application Reference

Wang IF et al. Proc Natl Acad Sci U S A 2012; 109 (37) : 15024-9 Autophagy activators rescue and alleviate pathogenesis of a mouse model with proteinopathies of the TAR DNA-binding protein 43.
Application : IHC
Reactivity : Mouse
Bose JK et al. J Biol Chem 2011; 286 (52) : 44441-8 Regulation of autophagy by neuropathological protein TDP-43.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 439