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TEAD4 antibody

Orthogonal
Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750
Anti-TEAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108750
Anti-TEAD4 antibody used in Western Blot (WB). GTX108750
Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750
Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750

Cat. No. GTX108750

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TEAD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Product Note

Cross-reactivity may occur with other family members.

Predict Reactivity

Mouse, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TEAD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952183

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TEA domain transcription factor 4 , EFTR-2 , RTEF1 , TCF13L1 , TEF-3 , TEF3 , TEFR-1 , hRTEF-1B

Cellular Localization

Nucleus

Background

This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750

GTX108750 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using TEAD4(GTX108750) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TEAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108750

GTX108750 ICC/IF Image

TEAD4 antibody detects TEAD4 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TEAD4 stained by TEAD4 antibody (GTX108750) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-TEAD4 antibody used in Western Blot (WB). GTX108750

GTX108750 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEAD4 antibody (GTX108750) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750

GTX108750 IHC-P Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2018. PMID: 30459528

Anti-TEAD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX108750

GTX108750 IHC-P Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2018. PMID: 30459528

REFERENCE

Application Reference

Huang Z et al. Onco Targets Ther 2021; 14 : 937-949 TEAD4 as a Prognostic Marker Promotes Cell Migration and Invasion of Urinary Bladder Cancer via EMT.
Application : WB, IHC-P
Reactivity : Human
Li CL et al. Anticancer Res 2020; 40 (10) : 5529-5538 Curcumol Suppresses Triple-negative Breast Cancer Metastasis by Attenuating Anoikis Resistance via Inhibition of Skp2-mediated Transcriptional Addiction.
Application : WB
Reactivity : Human
Zhang W et al. Cancer Cell Int 2018; 18 : 178 TEAD4 overexpression promotes epithelial-mesenchymal transition and associates with aggressiveness and adverse prognosis in head neck squamous cell carcinoma.
Application : IHC-P
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169