TEF1 antibody [GT13112]

Cat. No. GTX632126

Host	Mouse
Clonality	Monoclonal
Clone Name	GT13112
Isotype	IgG1
Application	WB, ICC/IF, IP
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX632126 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IP	1:100-1:500

Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888288

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TEA domain transcription factor 1 , AA , NTEF-1 , REF1 , TCF-13 , TCF13 , TEAD-1 , TEF-1

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq]

Database

Gene ID: 7003 TEAD1
UniProt: P28347 TEAD1

Research Area

DATA IMAGES

GTX632126 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632126 IP Image

Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (GTX632126).
Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX632126 ICC/IF Image

TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty