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TEF1 antibody [GT13112]

Anti-TEF1 antibody [GT13112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632126
Anti-TEF1 antibody [GT13112] used in Western Blot (WB). GTX632126
Anti-TEF1 antibody [GT13112] used in Immunoprecipitation (IP). GTX632126

Cat No. GTX632126

Host

Mouse

Clonality

Monoclonal

Clone Name

GT13112

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa NE

Predict Reactivity

Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human TEF1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888288

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TEA domain transcription factor 1 , AA , NTEF-1 , REF1 , TCF-13 , TCF13 , TEAD-1 , TEF-1

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TEF1 antibody [GT13112] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632126

GTX632126 ICC/IF Image

TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400.
Red: Phalloidin, a F-actin marker, diluted at 1:200.

Anti-TEF1 antibody [GT13112] used in Western Blot (WB). GTX632126

GTX632126 WB Image

TEF1 antibody [GT13112] detects TEF1 protein by western blot analysis. whole cell extracts and extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) at a dilution of 1:500.

Anti-TEF1 antibody [GT13112] used in Immunoprecipitation (IP). GTX632126

GTX632126 IP Image

Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (GTX632126).
Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

There are currently no references for TEF1 antibody [GT13112] (GTX632126). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TEF1 antibody [GT13112] (GTX632126). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169