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TEF1 antibody

Anti-TEF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32918
Anti-TEF1 antibody used in Western Blot (WB). GTX32918
Anti-TEF1 antibody used in ChIP assay (ChIP assay). GTX32918

Cat No. GTX32918

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
ChIP assay 1:50-1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TEA domain transcription factor 1 , AA , NTEF-1 , REF1 , TCF-13 , TCF13 , TEAD-1 , TEF-1

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-TEF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32918

GTX32918 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX32918 TEF1 antibody.

Anti-TEF1 antibody used in Western Blot (WB). GTX32918

GTX32918 WB Image

WB analysis of various sample lysates using GTX32918 TEF1 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-TEF1 antibody used in ChIP assay (ChIP assay). GTX32918

GTX32918 ChIP assay Image

ChIP analysis of HeLa cells using GTX32918 TEF1 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329