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TEF1 antibody

Anti-TEF1 antibody used in ChIP assay (ChIP assay). GTX32918
Anti-TEF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32918
Anti-TEF1 antibody used in Western Blot (WB). GTX32918

Cat. No. GTX32918

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all TEF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
ChIP assay 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TEA domain transcription factor 1 , AA , NTEF-1 , REF1 , TCF-13 , TCF13 , TEAD-1 , TEF-1

Cellular Localization

Nucleus

Background

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-TEF1 antibody used in ChIP assay (ChIP assay). GTX32918

GTX32918 ChIP assay Image

ChIP analysis of HeLa cell lysate using GTX32918 TEF1 antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-TEF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32918

GTX32918 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX32918 TEF1 antibody.

Anti-TEF1 antibody used in Western Blot (WB). GTX32918

GTX32918 WB Image

WB analysis of various sample lysates using GTX32918 TEF1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Zhou Y et al. Nat Commun 2024; 15 (1) : 1405 Cell softness renders cytotoxic T lymphocytes and T leukemic cells resistant to perforin-mediated killing.
Yamada S et al. Sci Adv 2023; 9 (15) : eade7047 TEAD1 trapping by the Q353R-Lamin A/C causes dilated cardiomyopathy.
Nie H et al. Theranostics 2021; 11 (8) : 3898-3915 The short isoform of PRLR suppresses the pentose phosphate pathway and nucleotide synthesis through the NEK9-Hippo axis in pancreatic cancer.
Application : ChIP assay
Reactivity : Human
Filandrov? R et al. Structure 2020; (Epub) Motif orientation matters: structural characterization of TEAD1 recognition of genomic DNA.
Application : IP
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399