APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5-1 μg/mL |
| 2.5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Colon Tissue Lysate(GTX27928)
Product Note
At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
TEM1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of the human TEM1.The immunogen is located within amino acids 40 - 90 of TEM1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD248 molecule , CD164L1 , TEM1
Cellular Localization
Membrane
Background
Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion. At least two isoforms of TEM1 are known to exist; this antibody recognizes only the larger isoform.
Database
Research Area
DATA IMAGES
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GTX85429 IHC-P Image
IHC-P analysis of formaldehyde fixed Human Colon Tissue using GTX85429 TEM1 antibody.
Antigen retreival : Heat mediation with a citrate buffer (pH6)
Dilution : 2.5μg/mL
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GTX85429 WB Image
WB analysis of various samples using GTX85429 TEM1 antibody.
Dilution : 0.5μg/mL
Loading : 15μg of lysates per lane
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GTX85429 WB Image
WB analysis of various samples using GTX85429 TEM1 antibody.
Dilution : 1μg/mL
Loading : 15μg of lysates per lane
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GTX85429 WB Image
WB analysis of human colon tissue lysate using GTX85429 TEM1 antibody.
Dilution : 0.5μg/mL(Lane 1) and 1μg/mL(Lane 2)
Loading : 15μg of lysates per lane
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GTX85429 IHC-P Image
IHC-P analysis of 4% PFA-fixed Human Colon Tissue using GTX85429 TEM1 antibody.
Dilution : 20μg/mL
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REFERENCE
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REVIEW
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