GeneTex
United States (US)

TEM8 antibody, C-term

GTX15724_1526_WB_18121410_440.jpg

Cat No. GTX15724

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.2-1μg/ml
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15724-PEP).

Specificity/Sensitivity

This antibody is expected to recognise variant 1 (NP_115584) only.

Predict Reactivity

Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RAPPPSRPPPRPSV, from the C Terminus of the protein sequence according to NP_115584.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Antxr Cell Adhesion Molecule 1,Atr,Gapo,Tem8,Antxr1

Cellular Localization

Type I membrane protein.

Background

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]

Database

DATA IMAGES
GTX15724_1526_WB_18121410_440.jpg

GTX15724 WB Image

WB analysis of human PBMC lysate using GTX15724 TEM8 antibody, C-term.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329