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TERT antibody [C2C3-2], C-term

Orthogonal
Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242
Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242
Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242

Cat. No. GTX124242

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TERT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

Human ESC , HepG2 nuclear extract

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TERT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170467

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

telomerase reverse transcriptase , CMM9 , DKCA2 , DKCB4 , EST2 , PFBMFT1 , TCS1 , TP2 , TRT , hEST2 , hTRT

Cellular Localization

Nucleus , Telomere

Background

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242

GTX124242 WB Image

Sample (20 ug of whole cell lysate)

A: human ESC

5% SDS PAGE

GTX124242 diluted at 1:1000

Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242

GTX124242 WB Image

Orthogonal

HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TERT antibody [C2C3-2], C-term (GTX124242) diluted at 1:500.

Anti-TERT antibody [C2C3-2], C-term used in Western Blot (WB). GTX124242

GTX124242 WB Image

Orthogonal

The data was published in the journal Cells in 2019. PMID: 31731674

REFERENCE

Application Reference

Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
Application : WB
Reactivity : Human
Wang S et al. Cell Death Differ 2020; (Epub) USP22 positively modulates ERα action via its deubiquitinase activity in breast cancer.
Application : WB
Reactivity : Human
Nevi L et al. Cells 2019; 8 (11) Cholest-4,6-Dien-3-One Promote Epithelial-To-Mesenchymal Transition (EMT) in Biliary Tree Stem/Progenitor Cell Cultures In Vitro.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169