GeneTex
United States (US)

TERT antibody [C2C3-2], C-term

GTX124242_40751_WB_18121410_707.jpg
GTX124242_40751_20160324_WB_Fraction_18121410_697.jpg

Cat No. GTX124242

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

127 kDa. ( Note )

Positive Control

human ESC , HepG2 nuclear extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TERT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Telomerase Reverse Transcriptase,Cmm9,Dkca2,Dkcb4,Est2,Pfbmft1,Tcs1,Tp2,Trt,Hest2,Htrt,Tert

Cellular Localization

Nucleus , Telomere

Background

Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124242_40751_WB_18121410_707.jpg

GTX124242 WB Image

Sample (20 ug of whole cell lysate)

A: human ESC

5% SDS PAGE

GTX124242 diluted at 1:1000

GTX124242_40751_20160324_WB_Fraction_18121410_697.jpg

GTX124242 WB Image

HepG2 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TERT antibody [C2C3-2], C-term (GTX124242) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169