APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:2000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.5% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide from human TERT protein (222-298 aa).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
telomerase reverse transcriptase , CMM9 , DKCA2 , DKCB4 , EST2 , PFBMFT1 , TCS1 , TP2 , TRT , hEST2 , hTRT
Cellular Localization
nucleoplasm , Nucleus , Cytoplasm , Nucleolus
Background
Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX03646 WB Image
WB analysis of HepG2 cell lysate using GTX03646 TERT antibody.
Dilution : 1:1000
|
|
|
GTX03646 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX03646 TERT antibody.
Dilution : 1:200
|
|
|
GTX03646 IHC-P Image
IHC-P analysis of human kidney tissue using GTX03646 TERT antibody. Negative control was used by secondary antibody only.
Antigen retrieval : Sodium citrate pH 6.0 was used for antigen retrieval (>98ºC, 20min)
Dilution : 1:200
|
REFERENCE
There are currently no references for TERT antibody (GTX03646). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for TERT antibody (GTX03646). Be the first to share your experience with this product.
