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TET1 antibody [GT1462]

KO/KD Orthogonal Overexpression
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420
Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

Cat No. GTX627420

Reference 20
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1462

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($359)
See all TET1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , DDDDK-human TET1-transfected 293T , NT2D1 , NT2D1 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11172316

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

KO/KD

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.
A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA
B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
6% SDS-PAGE
TET1 antibody [GT1462] (GTX627420) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420

GTX627420 ICC/IF Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

Orthogonal

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420

GTX627420 IP Image

Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (GTX627420).
Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

Overexpression

TET1 antibody detects TET1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody (GTX627420) dilution: 1:500

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Kim H et al. Genome Biol 2021; 22 (1) : 125 Ten-eleven translocation protein 1 modulates medulloblastoma progression.
Application : IP, ChIP assay
Reactivity : Mouse
Peng C et al. J Cell Physiol 2020; Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
Application : ChIP assay
Reactivity : Human
Skrajna A et al. Nucleic Acids Res 2020; 48 (17) : 9415-9432 Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding.
Application : WB
Reactivity : Human
Feng W et al. Mol Cell Biol 2020; 40 (13) DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
Application : WB
Reactivity : Human
Huang Y et al. Int J Mol Sci 2019; 20 (8) TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN-γ-Induced M1 Macrophage Polarization through the NF-κB Pathway in THP-1 Cells.
Application : WB
Reactivity : Human
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Submit a Reference

REVIEW

TET1 antibody [GT1462] Cat No. GTX627420

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31235851
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Date : Anonymous submitted on 26-Mar-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA, B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
Submit a Review
Application Reference
Kim H et al. Genome Biol 2021; 22 (1):125 Ten-eleven translocation protein 1 modulates medulloblastoma progression.
Application : IP, ChIP assay
Reactivity : Mouse
Peng C et al. J Cell Physiol 2020; Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes.
Application : ChIP assay
Reactivity : Human
Skrajna A et al. Nucleic Acids Res 2020; 48 (17):9415-9432 Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding.
Application : WB
Reactivity : Human
Feng W et al. Mol Cell Biol 2020; 40 (13) DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription.
Application : WB
Reactivity : Human
Chen LY et al. J Pathol 2019; (Epub) TET1 reprograms the epithelial ovarian cancer epigenome and reveals casein kinase 2α as a therapeutic target.
Huang Y et al. Int J Mol Sci 2019; 20 (8) TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN-γ-Induced M1 Macrophage Polarization through the NF-κB Pathway in THP-1 Cells.
Application : WB
Reactivity : Human
Su PH et al. Cancer Lett 2019; 450:53-62 TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.
Application : WB, IP, ChIP assay
Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.
Application : WB
Reactivity : Human
Wang KC et al. Oncol Lett 2018; 15 (1):278-284 Ten-eleven translocation 1 dysfunction reduces 5-hydroxymethylcytosine expression levels in gastric cancer cells.
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11):1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Application : WB
Reactivity : Mouse
Verma N et al. Nat Genet 2018; 50 (1):83-95 TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells.
Application : ChIP assay
Reactivity : Human
Li Q et al. Cell Prolif 2017; (Epub) FAM20C could be targeted by TET1 to promote odontoblastic differentiation potential of human dental pulp cells.
Application : WB, ChIP assay
Reactivity : Human
Jiang X et al. Nat Commun 2017; 8 (1):2099 Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.
Application : WB
Reactivity : Mouse
Pei YF et al. Biochim Biophys Acta 2016; 1862 (11):2177-2185 MiR-29a promotes cell proliferation and EMT in breast cancer by targeting ten eleven translocation 1.
Application : WB
Reactivity : Human
Rao LJ et al. Int J Oral Sci 2016; 8 (2):110-6 TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells.
Application : WB
Reactivity : Human
Yang YA et al. Nucleic Acids Res. 2016; FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function.
Application : ICC/IF, IP
Reactivity : Human
Loriot A et al. Epigenetics 2014; 9 (8):1163-71 A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.
Application : WB
Reactivity : Human
Naoko Irie et al. Cell 2014; 160 (43102):253-68 SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate.
Application : ICC/IF
Reactivity : Human
Li Q et al. J Endod 2014; Expression Features of DNA Methylcytosine Dioxygenase Ten-eleven Translocation 1 in Human Dental Pulp Cells.
Application : ICC/IF
Reactivity : Human
Mahaira LG et al. Stem Cells Dev 2014; (Epub) IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.
Application : ICC/IF, ChIP assay
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 359