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TET1 antibody [GT1462]

KO/KD Orthogonal Overexpression
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420
Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

Cat No. GTX627420

Reference 15
1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1462

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($359)
See all TET1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , DDDDK-human TET1-transfected 293T , NT2D1 , NT2D1 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

KO/KD

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.
A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA
B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
6% SDS-PAGE
TET1 antibody [GT1462] (GTX627420) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420

GTX627420 ICC/IF Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

Orthogonal

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420

GTX627420 IP Image

Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (GTX627420).
Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

Overexpression

TET1 antibody detects TET1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody (GTX627420) dilution: 1:500

REFERENCE

Application Reference

Chen LY et al. J Pathol 2019; (Epub) TET1 reprograms the epithelial ovarian cancer epigenome and reveals casein kinase 2α as a therapeutic target.
Huang Y et al. Int J Mol Sci 2019; 20 (8) TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN-γ-Induced M1 Macrophage Polarization through the NF-κB Pathway in THP-1 Cells.
Application : WB
Reactivity : Human
Su PH et al. Cancer Lett 2019; 450 : 53-62 TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.
Application : WB, IP, ChIP assay
Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.
Application : WB
Reactivity : Human
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11) : 1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Application : WB
Reactivity : Mouse
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REVIEW

TET1 antibody [GT1462] Cat No. GTX627420

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Date : Anonymous submitted on 26-Mar-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA, B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
Submit a Review
Application Reference
Chen LY et al. J Pathol 2019; (Epub) TET1 reprograms the epithelial ovarian cancer epigenome and reveals casein kinase 2α as a therapeutic target.
Huang Y et al. Int J Mol Sci 2019; 20 (8) TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN-γ-Induced M1 Macrophage Polarization through the NF-κB Pathway in THP-1 Cells.
Application : WB
Reactivity : Human
Su PH et al. Cancer Lett 2019; 450:53-62 TET1 promotes 5hmC-dependent stemness, and inhibits a 5hmC-independent epithelial-mesenchymal transition, in cervical precancerous lesions.
Application : WB, IP, ChIP assay
Reactivity : Human
Li M et al. Oncogene 2019; (Epub) Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2.
Application : WB
Reactivity : Human
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11):1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Application : WB
Reactivity : Mouse
Verma N et al. Nat Genet 2018; 50 (1):83-95 TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells.
Application : ChIP assay
Reactivity : Human
Li Q et al. Cell Prolif 2017; (Epub) FAM20C could be targeted by TET1 to promote odontoblastic differentiation potential of human dental pulp cells.
Application : WB, ChIP assay
Reactivity : Human
Jiang X et al. Nat Commun 2017; 8 (1):2099 Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.
Application : WB
Reactivity : Mouse
Pei YF et al. Biochim Biophys Acta 2016; 1862 (11):2177-2185 MiR-29a promotes cell proliferation and EMT in breast cancer by targeting ten eleven translocation 1.
Application : WB
Reactivity : Human
Rao LJ et al. Int J Oral Sci 2016; 8 (2):110-6 TET1 knockdown inhibits the odontogenic differentiation potential of human dental pulp cells.
Application : WB
Reactivity : Human
Yang YA et al. Nucleic Acids Res. 2016; FOXA1 potentiates lineage-specific enhancer activation through modulating TET1 expression and function.
Application : ICC/IF, IP
Reactivity : Human
Loriot A et al. Epigenetics 2014; 9 (8):1163-71 A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.
Application : WB
Reactivity : Human
Naoko Irie et al. Cell 2014; 160 (43102):253-68 SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate.
Application : ICC/IF
Reactivity : Human
Li Q et al. J Endod 2014; Expression Features of DNA Methylcytosine Dioxygenase Ten-eleven Translocation 1 in Human Dental Pulp Cells.
Application : ICC/IF
Reactivity : Human
Mahaira LG et al. Stem Cells Dev 2014; (Epub) IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.
Application : ICC/IF, ChIP assay
Reactivity : Human
SDS
PBS.pdf
Package List Price ($)
$ 359