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TET1 antibody [GT1462]

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420
Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420
Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420
Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

Cat No. GTX627420

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1462

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , DDDDK-human TET1-transfected 293T , NT2D1 , NT2D1 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_11172316

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.
A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA
B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
6% SDS-PAGE
TET1 antibody [GT1462] (GTX627420) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [GT1462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627420

GTX627420 ICC/IF Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

Anti-TET1 antibody [GT1462] used in Immunoprecipitation (IP). GTX627420

GTX627420 IP Image

Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (GTX627420).
Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-TET1 antibody [GT1462] used in Western Blot (WB). GTX627420

GTX627420 WB Image

TET1 antibody detects TET1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody (GTX627420) dilution: 1:500

Anti-TET1 antibody [GT1462] used in IHC (Paraffin sections) (IHC-P). GTX627420

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [GT1462] (GTX627420) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

TET1 antibody [GT1462] Cat No. GTX627420

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31235851
Date : Anonymous submitted on 26-Mar-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA, B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
Application Reference
SDS
PBS.pdf