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TET1 antibody [GT1462]

KO/KD Orthogonal Overexpression
GTX627420_40878_CT_WB_Knockdown_18121411_718.jpg
GTX627420_40878_IHC_18121411_840.jpg
GTX627420_40878_IFA_18121411_747.jpg
GTX627420_41386_20160721_WB_Fraction_18121411_134.jpg
GTX627420_40878_IP_18121411_365.jpg
GTX627420_41386_WB_B_18121411_360.jpg

Cat No. GTX627420

Host Mouse
Clonality Monoclonal
Clone Name GT1462
Isotype IgG2a
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , DDDDK-human TET1-transfected 293T , NT2D1 , NT2D1 nuclear extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CXXC6 antibody, FLJ10839 antibody, FLJ41442 antibody, KIAA1676 antibody, LCX antibody, bA119F7.1 antibody, TET1 antibody, methylcytosine dioxygenase TET1 antibody, leukemia-associated protein with a CXXC domain antibody, ten-eleven translocation 1 gene protein antibody, CXXC-type zinc finger protein 6 antibody, CXXC zinc finger 6 antibody, tet oncogene 1 antibody, CXXC finger 6 antibody, ten-eleven translocation-1 antibody

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES
GTX627420_40878_CT_WB_Knockdown_18121411_718.jpg

GTX627420 WB Image

KO/KD

TET1 antibody [GT1462] detects TET1 protein by western blot analysis.
A. 50 µg whole cell lysate/extract from 293T cells transfected with scramble siRNA
B. 50 µg whole cell lysate/extract from TET1-knockdowned 293T cells
6% SDS-PAGE
TET1 antibody [GT1462] (GTX627420) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX627420_40878_IHC_18121411_840.jpg

GTX627420 IHC-P Image

TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
TET1 antibody [GT1462] (GTX627420) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX627420_40878_IFA_18121411_747.jpg

GTX627420 ICC/IF Image

TET1 antibody [GT1462] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: TET1-transfected (right) or untransfected (left) 293T cells were fixed in 4% paraformaldehyde for 15 min. Green: TET1 protein stained by TET1 antibody (GTX627420) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

GTX627420_41386_20160721_WB_Fraction_18121411_134.jpg

GTX627420 WB Image

Orthogonal

NT2D1 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT1462] (GTX627420) diluted at 1:500.

GTX627420_40878_IP_18121411_365.jpg

GTX627420 IP Image

Immunoprecipitation of TET1 protein from NT2D1 whole cell extracts using 5 μg of TET1 antibody [GT1462] (GTX627420).
Western blot analysis was performed using TET1 antibody [GT1462] (GTX627420).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

GTX627420_41386_WB_B_18121411_360.jpg

GTX627420 WB Image

Overexpression

TET1 antibody detects TET1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody (GTX627420) dilution: 1:500

REFERENCE
REVIEW

TET1 antibody [GT1462] Cat No. GTX627420

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-Mar-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, overnightHr
Lane Description : A. 50 μg whole cell lysate/extract from 293T cells transfected with scramble siRNA, B. 50 μg whole cell lysate/extract from TET1-knockdowned 293T cells
Application Reference
SDS
PBS.pdf