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TET1 antibody [GT465]

Anti-TET1 antibody [GT465] used in Western Blot (WB). GTX629974
Anti-TET1 antibody [GT465] used in Western Blot (WB). GTX629974
Anti-TET1 antibody [GT465] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629974

Cat No. GTX629974

Host

Mouse

Clonality

Monoclonal

Clone Name

GT465

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($359),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

NT2D1 , NT2D1 nuclear extract , DDDDK-human TET1-transfected 293T , *M10

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888181

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [GT465] used in Western Blot (WB). GTX629974

GTX629974 WB Image

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT465] (GTX629974) diluted at 1:500.

Anti-TET1 antibody [GT465] used in Western Blot (WB). GTX629974

GTX629974 WB Image

TET1 antibody [GT465] detects TET1 protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody [GT465] (GTX629974) dilution: 1:1000

Anti-TET1 antibody [GT465] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629974

GTX629974 ICC/IF Image

TET1 antibody [GT465] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [GT465] (GTX629974) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

There are currently no references for TET1 antibody [GT465] (GTX629974). Be the first to share your publications with this product.

REVIEW

TET1 antibody [GT465] Cat No. GTX629974

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 24-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : M10 cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf