TET1 antibody [GT465]

Name: TET1 antibody [GT465]
Brand: GeneTex
SKU: GTX629974
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-TET1 antibody [GT465] used in Western Blot (WB). GTX629974

Anti-TET1 antibody [GT465] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629974

Cat. No. GTX629974

Host	Mouse
Clonality	Monoclonal
Clone Name	GT465
Isotype	IgG1
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX629974 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

DDDDK-human TET1-transfected 293T cells , NT2D1 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888181

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Gene ID: 80312 TET1
UniProt: Q8NFU7 TET1

Research Area

DATA IMAGES

GTX629974 WB Image

TET1 antibody [GT465] detects TET1 protein by Western blot analysis.
A. 30 μg 293T
B. 30 μg of DDDDK-human TET1-transfected 293T cells
5 % SDS-PAGE
TET1 antibody [GT465] (GTX629974) dilution: 1:1000

GTX629974 WB Image

NT2D1 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [GT465] (GTX629974) diluted at 1:500.

GTX629974 ICC/IF Image

TET1 antibody [GT465] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [GT465] (GTX629974) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

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REVIEW