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TET1 antibody [N1], N-term

KO/KD Orthogonal
Anti-TET1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX125888
Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888
Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888
Anti-TET1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125888
Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888
Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888

Cat. No. GTX125888

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

 Mouse
Package
100 μl ($399)
See all TET1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
IHC-Wm Assay dependent
IP Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

219 kDa. ( Note )

Positive Control

DDDDK-mouse TET1-transfected 293T cells

Product Note

Knockdown/Knockout validation was supported by references (PMID: 29513657)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse TET1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164485

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , 2510010B09Rik , AA517754 , BB001228 , Cxxc6 , D10Ertd17e , LCX , mKIAA1676

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX125888

GTX125888 IHC-P Image

TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N1], N-term (GTX125888) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888

GTX125888 WB Image

Western blot analysis of 293T cell are mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either GTX125888 (diluted at 1:2000) (A) or anti-tag (B) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888

GTX125888 WB Image

TET1 antibody detects TET1 protein by western blot analysis. Non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody (GTX125888) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125888

GTX125888 ICC/IF Image

KO/KD Orthogonal

The data was published in the journal Stem Cell Reports in 2018.PMID: 29576538

Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888

GTX125888 WB Image

Orthogonal

The data was published in the 2022 in Nucleic Acids Res. PMID: 35150568

Anti-TET1 antibody [N1], N-term used in Western Blot (WB). GTX125888

GTX125888 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016. PMID: 27449105

REFERENCE

Application Reference

Flores JC et al. iScience 2023; 26 (7) : 107170 Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cells.
van der Veer BK et al. Nucleic Acids Res 2023; 51 (11) : 5469-5498 Dual functions of TET1 in germ layer lineage bifurcation distinguished by genomic context and dependence on 5-methylcytosine oxidation.
Huang X et al. bioRxiv 2023; ZFP281 coordinates DNMT3 and TET1 for transcriptional and epigenetic control in pluripotent state transitions.
Application : WB, ChIP assay
Xin Huang et al. Cell Rep 2022; 39 (10) : 110928 A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.
Application : WB, IP, ChIP assay
Reactivity : Mouse
Stephanie Chrysanthou et al. Cells 2022; 11 (8) Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells.
Application : ChIP assay
Reactivity : Mouse
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REVIEW

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Application Reference
Flores JC et al. iScience 2023; 26 (7):107170 Tet2 regulates Sin3a recruitment at active enhancers in embryonic stem cells.
van der Veer BK et al. Nucleic Acids Res 2023; 51 (11):5469-5498 Dual functions of TET1 in germ layer lineage bifurcation distinguished by genomic context and dependence on 5-methylcytosine oxidation.
Huang X et al. bioRxiv 2023; ZFP281 coordinates DNMT3 and TET1 for transcriptional and epigenetic control in pluripotent state transitions.
Application : WB, ChIP assay
Xin Huang et al. Cell Rep 2022; 39 (10):110928 A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency.
Application : WB, IP, ChIP assay
Reactivity : Mouse
Stephanie Chrysanthou et al. Cells 2022; 11 (8) Tet1 Suppresses p21 to Ensure Proper Cell Cycle Progression in Embryonic Stem Cells.
Application : ChIP assay
Reactivity : Mouse
Chrysanthou S et al. Nucleic Acids Res 2022; (Epub) The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity.
Application : WB, IP
Reactivity : Mouse
Arand J et al. EMBO Rep 2021;:e53968 Tet enzymes are essential for early embryogenesis and completion of embryonic genome activation.
Application : IHC-Wm
Reactivity : Mouse
Huang Z et al. Sci Adv 2021; 7 (4) The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins.
Application : ChIP assay
Luo X et al. Genes Dev 2020; 34 (7月8日):598-618 Coordination of germ layer lineage choice by TET1 during primed pluripotency.
Kim KP et al. Sci Adv 2020; 6 (36) Reprogramming competence of OCT factors is determined by transactivation domains.
Application : WB
Senner CE et al. Stem Cell Reports 2020; (Epub) TET1 and 5-Hydroxymethylation Preserve the Stem Cell State of Mouse Trophoblast.
Application : ICC/IF
Bartoccetti M et al. Cell Rep 2020; 30 (7):2150-2169.e9 Regulatory Dynamics of Tet1 and Oct4 Resolve Stages of Global DNA Demethylation and Transcriptomic Changes in Reprogramming.
Application : WB
Reactivity : Mouse
Bai M et al. Oncogene 2018; (Epub) * sensitizes endometrial cancer cells to chemotherapy through idh1-induced nrf2 expression via an epigenetic mechanism.
Application : IHC-P
Chrysanthou S et al. Stem Cell Reports 2018; 10 (4):1355-1368 A Critical Role of TET1/2 Proteins in Cell-Cycle Progression of Trophoblast Stem Cells.
Application : ICC/IF, ChIP assay
Reactivity : Mouse
Hill PWS et al. Nature 2018; 555 (7696):392-396 Epigenetic reprogramming enables the transition from primordial germ cell to gonocyte.
Application : WB
Reactivity : Mouse
Coluccio A et al. Epigenetics Chromatin 2018; 11 (1):7 Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naive embryonic stem cells.
Application : ChIP assay
Reactivity : Mouse
Cui Y et al. Oncotarget 2016; 7 (40):64932-64941 miR-29 regulates Tet1 expression and contributes to early differentiation of mouse ESCs.
Application : WB
Reactivity : Mouse
Kang J et al. Proc Natl Acad Sci U S A 2015; 112 (31):E4236-45 Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399