GeneTex
United States (US)

TET1 antibody [N1], N-term

KO/KD
GTX125888_41584_20150226_WB_B_18121410_922.jpg
GTX125888_40772_WB_B_18121410_161.jpg
GTX125888_41059_IFA_18121410_457.jpg

Cat No. GTX125888

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

219 kDa. ( Note )

Positive Control

tagged-mTet1 , DDDDK-mouse TET1-transfected 293T

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID: 29513657)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of mouse TET1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tet Methylcytosine Dioxygenase 1,2510010B09Rik,Aa517754,Bb001228,Cxxc6,D10Ertd17E,Lcx,Mkiaa1676,Tet1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES
GTX125888_41584_20150226_WB_B_18121410_922.jpg

GTX125888 WB Image

TET1 antibody detects TET1 protein by western blot analysis. Non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody (GTX125888) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125888_40772_WB_B_18121410_161.jpg

GTX125888 WB Image

Western blot analysis of 293T cell are mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either GTX125888 (diluted at 1:2000) (A) or anti-tag (B) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX125888_41059_IFA_18121410_457.jpg

GTX125888 ICC/IF Image

TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 protein stained by TET1 antibody [N1], N-term (GTX125888) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf