GeneTex
United States (US)

TET1 antibody [N3C1]

KO/KD Orthogonal Overexpression
GTX124207_42690_20161201_WB_shRNA_watermark_18121410_246.jpg
GTX124207_42915_20170714_WB_18121410_524.jpg
GTX124207_41479_20151227_IHC-P_18121410_289.jpg
GTX124207_40751_WB_18121410_737.jpg
GTX124207_40751_CT_IHC_18121410_149.jpg
GTX124207_42915_20180907_WB_Fraction_18121410_698.jpg
GTX124207_42915_20180801_ICC_IF_B_18121410_782.jpg
GTX124207_41479_WB_B_18121410_521.jpg
GTX124207_40751_IHC_18121410_523.jpg

Cat No. GTX124207

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , 293T tagged-hTET1 , DDDDK-human TET1-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tet Methylcytosine Dioxygenase 1,Cxxc6,Lcx,Ba119F7.1,Tet1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES
GTX124207_42690_20161201_WB_shRNA_watermark_18121410_246.jpg

GTX124207 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124207_42915_20170714_WB_18121410_524.jpg

GTX124207 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124207_41479_20151227_IHC-P_18121410_289.jpg

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
TET1 antibody [N3C1] (GTX124207) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124207_40751_WB_18121410_737.jpg

GTX124207 WB Image

Overexpression

(A) western blot analysis of endogenous TET1 expression in HeLa (lane 1) and HepG2 cell (lane 2). (B) 293T cell are mock transfected (lane1 and 3) or with tagged-hTET1 (lane 2 and 4) for 24hrs, using either GTX124207 (diluted at 1:10000) (left) or anti-tag (right) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124207_40751_CT_IHC_18121410_149.jpg

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis.
Sample: Paraffin-embedded Human normal prostate tissue.
TET1 antibody [N3C1] (GTX124207) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124207_42915_20180907_WB_Fraction_18121410_698.jpg

GTX124207 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124207_42915_20180801_ICC_IF_B_18121410_782.jpg

GTX124207 ICC/IF Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX124207_41479_WB_B_18121410_521.jpg

GTX124207 WB Image

Overexpression

TET1 antibody [N3C1] detects TET1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5% SDS-PAGE
TET1 antibody [N3C1] (GTX124207) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124207_40751_IHC_18121410_523.jpg

GTX124207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

TET1 antibody [N3C1] Cat No. GTX124207

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 14-Oct-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human pancreatic ductal adenocarcinoma
Fixation : 10% paraformaldehyde
Primary Antibody : 1:200, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf