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TET2 antibody [N2-2], N-term

KO/KD Overexpression
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205
Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

Cat. No. GTX124205

Reference 16

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TET2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

human TET2-transfected 293T cells

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.55 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166461

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 2 , KIAA1546 , MDS

Background

The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

KO/KD

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205

GTX124205 ICC/IF Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205

GTX124205 ChIP assay Image

Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
ChIP experiment and primer designs are based on Nat Commun. 2013;4:2166.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205

GTX124205 IP Image

Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205).
Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Overexpression

TET2 antibody detects TET2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody (GTX124205) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tung Chao et al. Autophagy 2021; 17 (11) : 3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
Application : WB
Reactivity : Human
Shirui Huang et al. Cancer Cell Int 2021; 21 (1) : 241 miR-190 promotes malignant transformation and?progression of human urothelial cells through CDKN1B/p27 inhibition.
Application : WB
Reactivity : Human
Yamashita H et al. Virchows Arch 2021; (Epub) Epigenetic upregulation of TET2 is an independent poor prognostic factor for intrahepatic cholangiocarcinoma.
Application : IHC-P
Reactivity : Human
Chen W et al. Cell Rep 2021; 37 (5) : 109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
Application : WB
Reactivity : Mouse
Williamson SM et al. J Insect Physiol 2021; 134 : 104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
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REVIEW

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Application Reference
Tung Chao et al. Autophagy 2021; 17 (11):3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
Application : WB
Reactivity : Human
Shirui Huang et al. Cancer Cell Int 2021; 21 (1):241 miR-190 promotes malignant transformation and?progression of human urothelial cells through CDKN1B/p27 inhibition.
Application : WB
Reactivity : Human
Yamashita H et al. Virchows Arch 2021; (Epub) Epigenetic upregulation of TET2 is an independent poor prognostic factor for intrahepatic cholangiocarcinoma.
Application : IHC-P
Reactivity : Human
Chen W et al. Cell Rep 2021; 37 (5):109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
Application : WB
Reactivity : Mouse
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Wu SL et al. Genomics Proteomics Bioinformatics 2021; Genome-wide 5-hydroxymethylcytosine Profiling Analysis Identifies MAP7D1 as A Novel Regulator of Lymph Node Metastasis in Breast Cancer.
Application : IHC-P
Reactivity : Human
Huang F et al. Aging (Albany NY) 2020; 12 (17):16759-16774 HDAC4 inhibition disrupts TET2 function in high-risk MDS and AML.
Application : WB
Reactivity : Mouse
Gu Y et al. FASEB J 2020; (Epub) Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.
Application : IHC-P
Reactivity : Human
Xu Y et al. Front Endocrinol (Lausanne) 2020; 11:361 Changes in DNA 5-Hydroxymethylcytosine Levels and the Underlying Mechanism in Non-functioning Pituitary Adenomas.
Application : IHC-P
Reactivity : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Chatterjee B et al. Biochim Biophys Acta Gene Regul Mech 2018; 1861 (11):1048-1061 DNA Demethylation by DNMT3A and DNMT3B in vitro and of Methylated Episomal DNA in Transiently Transfected Cells.
Application : WB
Reactivity : Mouse
Sun J et al. Cell Stem Cell 2018; 23 (3):355-369.e9 SIRT1 Activation Disrupts Maintenance of Myelodysplastic Syndrome Stem and Progenitor Cells by Restoring TET2 Function.
Application : WB
Zhou Y et al. Cell Physiol Biochem 2018; 45 (6):2548-2559 Ginsenoside 20(S)-Rg3 Inhibits the Warburg Effect Via Modulating DNMT3A/ MiR-532-3p/HK2 Pathway in Ovarian Cancer Cells.
Application : WB
Reactivity : Human
Ancey P et al. Stem Cell Reporters 2017; 9:264-278 TET-Catalyzed 5-Hydroxymethylation Precedes HNF4A Promoter Choice during Differentiation of Bipotent Liver Progenitors
Application : ChIP assay
Reactivity : Human
Ni K et al. Hum Reprod 2016; (Epub) TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
Application : WB, IHC-P
Reactivity : Human
Mahaira LG et al. Stem Cells Dev 2014; (Epub) IGF2BP1 expression in human mesenchymal stem cells significantly affects their proliferation and is under the epigenetic control of TET1/2 demethylases.
Application : ICC/IF
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169