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TET2 antibody [N2-2], N-term

Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205
Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205
Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205
Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

Cat No. GTX124205

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , DDDDK-human TET2-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166461

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 2 , KIAA1546 , MDS

Background

The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES

Anti-TET2 antibody [N2-2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124205

GTX124205 ICC/IF Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET2 protein stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET2 antibody [N2-2], N-term used in ChIP assay (ChIP assay). GTX124205

GTX124205 ChIP assay Image

Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
ChIP experiment and primer designs are based on Nat Commun. 2013;4:2166.

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

TET2 antibody detects TET2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody (GTX124205) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in Western Blot (WB). GTX124205

GTX124205 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in Immunoprecipitation (IP). GTX124205

GTX124205 IP Image

Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 μg of TET2 antibody [N2-2], N-term (GTX124205).
Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-TET2 antibody [N2-2], N-term used in IHC (Paraffin sections) (IHC-P). GTX124205

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf