GeneTex
United States (US)

TET2 antibody [N2-2], N-term

GTX124205_41466_20150410_IFA_18121410_729.jpg
GTX124205_41556_20181123_WB_KO_watermark_19011519_143.jpg
GTX124205_41466_IHC_2_18121410_993.jpg
GTX124205_41556_ChIP_18121410_188.jpg
GTX124205_41466_WB_B_18121410_134.jpg
GTX124205_41466_WB_18121410_707.jpg
GTX124205_41466_IHC_18121410_897.jpg
GTX124205_41556_20170316_IP_18121410_686.jpg

Cat No. GTX124205

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , DDDDK-human TET2-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tet Methylcytosine Dioxygenase 2,Kiaa1546,Mds,Tet2

Background

The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
GTX124205_41466_20150410_IFA_18121410_729.jpg

GTX124205 ICC/IF Image

TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET2 protein stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX124205_41556_20181123_WB_KO_watermark_19011519_143.jpg

GTX124205 WB Image

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX124205_41466_IHC_2_18121410_993.jpg

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded cervical carcinoma.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124205_41556_ChIP_18121410_188.jpg

GTX124205 ChIP assay Image

Cross-linked ChIP was performed with U2OS chromatin extract and 5 μg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
ChIP experiment and primer designs are based on Nat Commun. 2013;4:2166.

GTX124205_41466_WB_B_18121410_134.jpg

GTX124205 WB Image

TET2 antibody detects TET2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells
5% SDS-PAGE
TET2 antibody (GTX124205) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124205_41466_WB_18121410_707.jpg

GTX124205 WB Image

TET2 antibody [N2-2], N-term detects TET2 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
5% SDS-PAGE
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124205_41466_IHC_18121410_897.jpg

GTX124205 IHC-P Image

TET2 antibody [N2-2], N-term detects TET2 protein on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124205_41556_20170316_IP_18121410_686.jpg

GTX124205 IP Image

Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 µg of TET2 antibody [N2-2], N-term (GTX124205).
Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169