GeneTex
United States (US)

TET2 antibody

GTX124227_42760_20181123_WB_KO_watermark_19011519_990.jpg
GTX124227_42760_20170223_WB_18121410_430.jpg

Cat No. GTX124227

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

224 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tet Methylcytosine Dioxygenase 2,Kiaa1546,Mds,Tet2

Database

Research Area

DATA IMAGES
GTX124227_42760_20181123_WB_KO_watermark_19011519_990.jpg

GTX124227 WB Image

Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody (GTX124227) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by www.edigene.com

GTX124227_42760_20170223_WB_18121410_430.jpg

GTX124227 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody (GTX124227) diluted at 1:500.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169