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TET3 antibody [C3], C-term

Overexpression
Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

Cat. No. GTX121453

Reference 26
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Bovine, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all TET3 products
PRODUCT

Summary

TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723106

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 3 , hCG_40738

Cellular Localization

Nucleus,Cytoplasm

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453

GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453

GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

GTX121453 IHC-P Image

TET3 antibody [C3], C-term detects TET3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

REFERENCE

Application Reference

Ciesielski P et al. Int J Mol Sci 2021; 22 (24) TET3- and OGT-Dependent Expression of Genes Involved in Epithelial-Mesenchymal Transition in Endometrial Cancer.
Chen W et al. Cell Rep 2021; 37 (5) : 109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
Application : WB
Reactivity : Mouse
Aggarwal RK et al. Proc Natl Acad Sci U S A 2021; 118 (39) Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.
Application : WB
Reactivity : Human
Nishikawa K et al. EMBO Rep 2021; : e53035 Osteoclasts adapt to physioxia perturbation through DNA demethylation.
Application : WB
Reactivity : Mouse
Kawakubo-Yasukochi T et al. Mol Metab 2021; 54 : 101360 Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice.
Application : ChIP assay
Reactivity : Mouse
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Submit a Reference

REVIEW

TET3 antibody [C3], C-term Cat. No. GTX121453

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Endocrinol in 2020.PMID: 32089682
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 31089260
Submit a Review
Application Reference
Ciesielski P et al. Int J Mol Sci 2021; 22 (24) TET3- and OGT-Dependent Expression of Genes Involved in Epithelial-Mesenchymal Transition in Endometrial Cancer.
Chen W et al. Cell Rep 2021; 37 (5):109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
Application : WB
Reactivity : Mouse
Aggarwal RK et al. Proc Natl Acad Sci U S A 2021; 118 (39) Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.
Application : WB
Reactivity : Human
Nishikawa K et al. EMBO Rep 2021;:e53035 Osteoclasts adapt to physioxia perturbation through DNA demethylation.
Application : WB
Reactivity : Mouse
Kawakubo-Yasukochi T et al. Mol Metab 2021; 54:101360 Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice.
Application : ChIP assay
Reactivity : Mouse
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Gu Y et al. FASEB J 2020; (Epub) Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.
Application : IHC-P
Reactivity : Human
Yu S et al. Int J Endocrinol 2020; 2020:3909610 TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion.
Application : WB
Reactivity : Human
Xu Y et al. Cell Rep 2020; 30 (5):1310-1318.e5 A Positive Feedback Loop of TET3 and TGF-β1 Promotes Liver Fibrosis.
Application : WB
Reactivity : Mouse
Li Q et al. Mol Reprod Dev 2019; (Epub) DNA methylation regulated by ascorbic acids in yak preimplantation embryo helps to improve blastocyst quality.
Application : IHC-Wm
Cao T et al. Oncogene 2019; (Epub) H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas.
Application : WB
Reactivity : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Selimoglu-Buet D et al. Nat Commun 2018; 9 (1):5455 A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset.
Application : WB
Reactivity : Human
Liu C et al. Int J Neuropsychopharmacol 2017; (Epub) Retrieval-induced Upregulation of Tet3 in Pyramidal Neurons of the Dorsal Hippocampus Mediates Cocaine-Associated Memory Reconsolidation.
Application : WB
Lu F et al. J Virol 2017; (Epub) Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus.
Application : WB
Reactivity : Human
Ni K et al. Hum Reprod 2016; (Epub) TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
Application : WB, IHC-P
Reactivity : Human
Tampe B et al. EBioMedicine 2015; 2 (1):19-36 Induction of Tet3-dependent Epigenetic Remodeling by Low-dose * Attenuates Progression of Chronic Kidney Disease.
Application : WB
Reactivity : Mouse
Xu X et al. Cardiovasc Res 2015; 105 (3):279-91 Epigenetic balance of aberrant Rasal1 promoter methylation and hydroxymethylation regulates cardiac fibrosis.
Application : ChIP assay
Reactivity : Human
Ichimura N et al. Cancer Prev Res (Phila) 2015; 8 (8):702-11 Aberrant TET1 Methylation Closely Associated with CpG Island Methylator Phenotype in Colorectal Cancer.
Application : WB
Reactivity : Human
Kang J et al. Proc Natl Acad Sci U S A 2015; 112 (31):E4236-45 Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
Zhang Q et al. Nature 2015; (Epub) Tet2 is required to resolve inflammation by recruiting Hdac2 to specifically repress IL-6.
Application : WB
Reactivity : Mouse
Loriot A et al. Epigenetics 2014; 9 (8):1163-71 A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.
Application : WB
Reactivity : Human
Rampal R et al. Cell Rep 2014; DNA Hydroxymethylation Profiling Reveals that WT1 Mutations Result in Loss of TET2 Function in Acute Myeloid Leukemia.
Application : WB
Reactivity : Human
Page-Lariviere F et al. Cell Reprogram 2014; 16 (1):40-53 Spatiotemporal expression of DNA demethylation enzymes and histone demethylases in bovine embryos.
Application : WB, IHC-Wm
Reactivity : Bovine
Cheng J et al. Cell Rep 2013; 5 (2):471-81 An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis.
Application : WB
Reactivity : Human
Xu Y et al. Cell 2012; 151 (6):1200-13 Tet3 CXXC domain and dioxygenase activity cooperatively regulate key genes for Xenopus eye and neural development.
Application : WB
Reactivity : Xenopus laevis
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169