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TET3 antibody [C3], C-term

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

Cat No. GTX121453

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

Human, Mouse, Bovine, Xenopus laevis
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

Positive Control

Flag-human TET3 transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723106

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tet methylcytosine dioxygenase 3 , hCG_40738

Cellular Localization

Nucleus,Cytoplasm

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453

GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453

GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

GTX121453 IHC-P Image

TET3 antibody [C3], C-term detects TET3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

REFERENCE

REVIEW

TET3 antibody [C3], C-term Cat No. GTX121453

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Endocrinol in 2020.PMID: 32089682
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019.PMID: 31089260
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf