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TET3 antibody [C3], C-term

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

Cat. No. GTX121453

Reference 29
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay

Reactivity

 Human, Mouse, Bovine, Xenopus laevis
Package
100 μl ($399),
25 μl ($169)
See all TET3 products
PRODUCT

Summary

TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

Predict Reactivity

Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10723106

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 3 , hCG_40738

Cellular Localization

Nucleus,Cytoplasm

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453

GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453

GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

GTX121453 IHC-P Image

TET3 antibody [C3], C-term detects TET3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:500.
Antigen Retrieval: Trilogy™M (EDTA based) buffer, 15min

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Ye B et al. Cell Mol Immunol 2022; 19 (5) : 619-633 Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.
Xie D et al. J Clin Invest 2022; 132 (19) TET3 epigenetically controls feeding and stress response behaviors via AGRP neurons.
Cheng S et al. Cell 2022; 185 (17) : 3169-3185.e20 The intrinsic and extrinsic effects of TET proteins during gastrulation.
Ciesielski P et al. Int J Mol Sci 2021; 22 (24) TET3- and OGT-Dependent Expression of Genes Involved in Epithelial-Mesenchymal Transition in Endometrial Cancer.
Kawakubo-Yasukochi T et al. Mol Metab 2021; 54 : 101360 Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice.
Application : ChIP assay
Reactivity : Mouse
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Submit a Reference

REVIEW

TET3 antibody [C3], C-term Cat. No. GTX121453

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Endocrinol in 2020.PMID: 32089682
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncogene in 2019. PMID: 31089260
Submit a Review
Application Reference
Ye B et al. Cell Mol Immunol 2022; 19 (5):619-633 Induction of functional neutrophils from mouse fibroblasts by thymidine through enhancement of Tet3 activity.
Xie D et al. J Clin Invest 2022; 132 (19) TET3 epigenetically controls feeding and stress response behaviors via AGRP neurons.
Cheng S et al. Cell 2022; 185 (17):3169-3185.e20 The intrinsic and extrinsic effects of TET proteins during gastrulation.
Ciesielski P et al. Int J Mol Sci 2021; 22 (24) TET3- and OGT-Dependent Expression of Genes Involved in Epithelial-Mesenchymal Transition in Endometrial Cancer.
Chen W et al. Cell Rep 2021; 37 (5):109912 Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring.
Application : WB
Reactivity : Mouse
Aggarwal RK et al. Proc Natl Acad Sci U S A 2021; 118 (39) Functional succinate dehydrogenase deficiency is a common adverse feature of clear cell renal cancer.
Application : WB
Reactivity : Human
Nishikawa K et al. EMBO Rep 2021;:e53035 Osteoclasts adapt to physioxia perturbation through DNA demethylation.
Application : WB
Reactivity : Mouse
Kawakubo-Yasukochi T et al. Mol Metab 2021; 54:101360 Hepatic glycogenolysis is determined by maternal high-calorie diet via methylation of Pygl and it is modified by oteocalcin administration in mice.
Application : ChIP assay
Reactivity : Mouse
Williamson SM et al. J Insect Physiol 2021; 134:104298 Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.
Application : WB
Gu Y et al. FASEB J 2020; (Epub) Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.
Application : IHC-P
Reactivity : Human
Yu S et al. Int J Endocrinol 2020; 2020:3909610 TET1 is a Tumor Suppressor That Inhibits Papillary Thyroid Carcinoma Cell Migration and Invasion.
Application : WB
Reactivity : Human
Xu Y et al. Cell Rep 2020; 30 (5):1310-1318.e5 A Positive Feedback Loop of TET3 and TGF-β1 Promotes Liver Fibrosis.
Application : WB
Reactivity : Mouse
Li Q et al. Mol Reprod Dev 2019; (Epub) DNA methylation regulated by ascorbic acids in yak preimplantation embryo helps to improve blastocyst quality.
Application : IHC-Wm
Cao T et al. Oncogene 2019; (Epub) H19 lncRNA identified as a master regulator of genes that drive uterine leiomyomas.
Application : WB
Reactivity : Human
Pei YF et al. Am J Pathol 2019; (Epub) Methyl-CpG Binding Domain Protein 2 Inhibits the Malignant Characteristic of Lung Adenocarcinoma through the Epigenetic Modulation of 10 to 11 Translocation 1 and miR-200s.
Application : WB
Reactivity : Human
Selimoglu-Buet D et al. Nat Commun 2018; 9 (1):5455 A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset.
Application : WB
Reactivity : Human
Liu C et al. Int J Neuropsychopharmacol 2017; (Epub) Retrieval-induced Upregulation of Tet3 in Pyramidal Neurons of the Dorsal Hippocampus Mediates Cocaine-Associated Memory Reconsolidation.
Application : WB
Lu F et al. J Virol 2017; (Epub) Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus.
Application : WB
Reactivity : Human
Ni K et al. Hum Reprod 2016; (Epub) TET enzymes are successively expressed during human spermatogenesis and their expression level is pivotal for male fertility.
Application : WB, IHC-P
Reactivity : Human
Tampe B et al. EBioMedicine 2015; 2 (1):19-36 Induction of Tet3-dependent Epigenetic Remodeling by Low-dose * Attenuates Progression of Chronic Kidney Disease.
Application : WB
Reactivity : Mouse
Xu X et al. Cardiovasc Res 2015; 105 (3):279-91 Epigenetic balance of aberrant Rasal1 promoter methylation and hydroxymethylation regulates cardiac fibrosis.
Application : ChIP assay
Reactivity : Human
Ichimura N et al. Cancer Prev Res (Phila) 2015; 8 (8):702-11 Aberrant TET1 Methylation Closely Associated with CpG Island Methylator Phenotype in Colorectal Cancer.
Application : WB
Reactivity : Human
Kang J et al. Proc Natl Acad Sci U S A 2015; 112 (31):E4236-45 Simultaneous deletion of the methylcytosine oxidases Tet1 and Tet3 increases transcriptome variability in early embryogenesis.
Zhang Q et al. Nature 2015; (Epub) Tet2 is required to resolve inflammation by recruiting Hdac2 to specifically repress IL-6.
Application : WB
Reactivity : Mouse
Loriot A et al. Epigenetics 2014; 9 (8):1163-71 A novel cancer-germline transcript carrying pro-metastatic miR-105 and TET-targeting miR-767 induced by DNA hypomethylation in tumors.
Application : WB
Reactivity : Human
Rampal R et al. Cell Rep 2014; DNA Hydroxymethylation Profiling Reveals that WT1 Mutations Result in Loss of TET2 Function in Acute Myeloid Leukemia.
Application : WB
Reactivity : Human
Page-Lariviere F et al. Cell Reprogram 2014; 16 (1):40-53 Spatiotemporal expression of DNA demethylation enzymes and histone demethylases in bovine embryos.
Application : WB, IHC-Wm
Reactivity : Bovine
Cheng J et al. Cell Rep 2013; 5 (2):471-81 An extensive network of TET2-targeting MicroRNAs regulates malignant hematopoiesis.
Application : WB
Reactivity : Human
Xu Y et al. Cell 2012; 151 (6):1200-13 Tet3 CXXC domain and dioxygenase activity cooperatively regulate key genes for Xenopus eye and neural development.
Application : WB
Reactivity : Xenopus laevis
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
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