GeneTex
  • Country / Location Selection

United States (US)

TET3 antibody [C3], C-term

Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

Cat No. GTX121453

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay
Reactivity Human, Mouse, Bovine, Xenopus laevis
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

Positive Control

Flag-human TET3 transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tet methylcytosine dioxygenase 3,hCG_40738

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM]

Database

DATA IMAGES
Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453

GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TET3 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX121453

GTX121453 IHC-P Image

TET3 antibody [C3], C-term detects TET3 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
TET3 antibody [C3], C-term (GTX121453) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453

GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453

GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
REVIEW
There are currently no reviews for TET3 antibody [C3], C-term (GTX121453). Be the first to share your experience with this product.
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169