TET3 antibody [C3], C-term

Cat. No. GTX121453
References 39
Cat. No.  GTX121453
Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
GTX121453 IP Image

TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 μg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

1 / 6
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
GTX121453 ICC/IF Image

TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

2 / 6
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

3 / 6
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
GTX121453 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

4 / 6
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
GTX121453 WB Image

The data was published in the journal Oncogene in 2019. PMID: 31089260

5 / 6
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
GTX121453 WB Image

The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682

6 / 6
Anti-TET3 antibody [C3], C-term used in Immunoprecipitation (IP). GTX121453
Anti-TET3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
Anti-TET3 antibody [C3], C-term used in Western Blot (WB). GTX121453
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF IHC-Wm IP ChIP assay
  • Reactivity
    Human, Mouse, Bovine, Xenopus laevis
Summary
TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.
See all TET3 products

Ask