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TET3 antibody [N3C1], Internal

Anti-TET3 antibody [N3C1], Internal used in Western Blot (WB). GTX121452
Anti-TET3 antibody [N3C1], Internal used in Western Blot (WB). GTX121452

Cat. No. GTX121452

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all TET3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

179 kDa. ( Note )

Positive Control

Flag-human TET3-transfected 293T cells

Predict Reactivity

Mouse, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731957

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 3 , hCG_40738

Cellular Localization

Nucleus,Cytoplasm

Background

Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TET3 antibody [N3C1], Internal used in Western Blot (WB). GTX121452

GTX121452 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [N3C1], Internal (GTX121452) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET3 antibody [N3C1], Internal used in Western Blot (WB). GTX121452

GTX121452 WB Image

The data was published in the journal Clin Epigenetics in 2016. PMID: 26816554

REFERENCE

Application Reference

Feng Wan et al. Am J Transl Res 2021; 13 (3) : 1233-1244 TET2 mediated demethylation is involved in the protective effect of triptolide on podocytes.
Application : WB
Reactivity : Human
Huang Y et al. Clin Epigenetics 2016; 8 : 9 Loss of nuclear localization of TET2 in colorectal cancer.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169