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TFE3 antibody [GT1317]

Recombinant antibody Orthogonal
Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229
Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229
Anti-TFE3 antibody [GT1317] used in IHC (Paraffin sections) (IHC-P). GTX03229
Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229

Cat. No. GTX03229

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1317

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all TFE3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Observed MW

89/72 kDa. The observed M.W. is based on the following publication. PMID: 21209915

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human TFE3.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor binding to IGHM enhancer 3 , RCCP2 , RCCX1 , TFEA , bHLHe33

Cellular Localization

Nucleus

Background

This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229

GTX03229 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFE3 antibody [GT1317] (GTX03229) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 21209915

Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229

GTX03229 WB Image

WB analysis of HeLa whole cell lysate using GTX03229 TFE3 antibody [GT1317].
Dilution : 1:1000
Loading : 25μg per lane

Anti-TFE3 antibody [GT1317] used in IHC (Paraffin sections) (IHC-P). GTX03229

GTX03229 IHC-P Image

IHC-P analysis of human esophageal cancer section using GTX03229 TFE3 antibody [GT1317].
Dilution : 1:100

Anti-TFE3 antibody [GT1317] used in Western Blot (WB). GTX03229

GTX03229 WB Image

WB analysis of various samples using GTX03229 TFE3 antibody [GT1317].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 439