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TFEB antibody [HL1065]

Recombinant antibody Comparison Orthogonal
Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089
Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089
Anti-TFEB antibody [HL1065] used in IHC (Paraffin sections) (IHC-P). GTX636089

Cat. No. GTX636089

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1065

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all TFEB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TFEB. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909942

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

transcription factor EB , ALPHATFEB , BHLHE35 , TCFEB

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089

GTX636089 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TFEB antibody [HL1065] (GTX636089) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-TFEB antibody [HL1065] used in Western Blot (WB). GTX636089

GTX636089 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFEB antibody [HL1065] (GTX636089) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TFEB antibody [HL1065] used in IHC (Paraffin sections) (IHC-P). GTX636089

GTX636089 IHC-P Image

TFEB antibody [HL1065] detects TFEB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
TFEB stained by TFEB antibody [HL1065] (GTX636089) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations