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TFEC antibody [N1], N-term

Anti-TFEC antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104824
Anti-TFEC antibody [N1], N-term used in Western Blot (WB). GTX104824

Cat. No. GTX104824

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all TFEC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TFEC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038109

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

transcription factor EC , TCFEC , TFE-C , TFEC-L , TFECL , bHLHe34 , hTFEC-L

Cellular Localization

Nucleus

Background

Transcriptional regulator that acts as a repressor or an activator. Acts as a transcriptional repressor on minimal promoter containing element F (that includes an E-box sequence). Binds to element F in an E-box sequence-specific manner. Acts as a transcriptional transactivator on the proximal promoter region of the tartrate-resistant acid phosphatase (TRAP) E-box containing promoter (By similarity). Collaborates with MITF in target gene activation (By similarity). Acts as a transcriptional repressor on minimal promoter containing mu E3 enhancer sequence (By similarity). Binds to mu E3 DNA sequence of the immunoglobulin heavy-chain gene enhancer (By similarity). Binds DNA in a homo- or heterodimeric form.

Database

Research Area

DATA IMAGES

Anti-TFEC antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104824

GTX104824 ICC/IF Image

TFEC antibody [N1], N-term detects TFEC protein at nucleus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TFEC protein stained by TFEC antibody [N1], N-term (GTX104824) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-TFEC antibody [N1], N-term used in Western Blot (WB). GTX104824

GTX104824 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
TFEC antibody
GTX104824 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169